刺五加叶的HPLC-UV和ESI-MS指纹图谱研究  

The Fingerprints of Leaves of Acanthopanax Senticosus by HPLC-UV and ESI-MS

作　　者：周慧[1,2] 宋凤瑞[1] 刘志强[1] 郑毅男[2] 刘淑莹[1] 

机构地区：[1]中国科学院长春应用化学研究所,吉林长春130022 [2]吉林农业大学,吉林长春130118

出　　处：《质谱学报》2008年第6期321-326,共6页Journal of Chinese Mass Spectrometry Society

基　　金：国家自然科学基金项目(No.30472134);吉林省科技发展计划重点项目(No.20060902)资助

摘　　要：对12批不同来源的刺五加叶提取物进行指纹图谱研究,并利用ESI-MS指纹图谱鉴别刺五加叶与山楂叶。分别采用高效液相色谱(HPLC-UV)和电喷雾电离质谱(ESI-MS)测定12批不同来源的刺五加叶提取物,利用ESI-MS技术测定刺五加叶与山楂叶提取物,得到了分离度、精密度和重现性均较好的刺五加叶HPLC-UV及ESI-MS指纹图谱;同时,利用刺五加叶与山楂叶ESI-MS指纹图谱的差异,成功鉴别了二者,可为刺五加叶药材的质量控制提供参考。The extracts of leaves of Acanthopanax Senticosus were studied by HPLC-UV and ESI-MS. 12 samples from different sources were detected by HPLC-UV and ESI-MS. Perfect fingerprints were obtained. Meanwhile, leaves of Acanthopanax Senticosus and leaves of Folurn crataegi were identified successfully by using the fingerprints of ESI-MS. The method is simple, accurate. It can be used for the quality control of leaves of Acanthopanax Senticosus.

关 键 词：刺五加叶 指纹图谱 HPLC-UV ESI-MS 

分 类 号：O657.63[理学—分析化学] O657.7[理学—化学]