克氏原螯虾SOD同工酶的组织特异性研究  

Study on the Tissue Specificity of SOD Isozyme in Procambarus clarkia

作　　者：崔雪[1,2] 张引[2] 黄孝湘[2] 徐东生[2] 吕邦玉[2] 

机构地区：[1]华中农业大学水产学院,湖北武汉430074 [2]孝感学院生命科学技术学院,湖北孝感432100

出　　处：《河北农业科学》2008年第11期55-56,84,共3页Journal of Hebei Agricultural Sciences

基　　金：孝感学院校内项目

摘　　要：了解克氏原螯虾体内不同组织中SOD同工酶的表达情况,以期为克氏原螯虾生化遗传研究提供参考。采用聚丙烯酰胺凝胶垂直平板电泳法,研究克氏原螯虾4种组织或器官(肌肉、眼球、肝胰腺和心脏)中超氧化物歧化酶(SOD)同工酶的表达情况。结果表明:肌肉、眼球和心脏中只有1条带(Sod-1),肝胰腺中表达出2条带(Sod-1和Sod-2)。肝胰腺中的Sod-1表达最强,Sod-2只在肝胰腺中表达,肌肉中Sod1的表达为单态。不同组织中SOD同工酶的表达存在差异,表现出明显的组织特异性。Expressions of SOD isozyme in different tissues of Procambarus clarkia were comprehended, in order to provide references for the study on inheritance of Procambarus clarkia. By polyacrylamide gel disc vertical electrophoresis, expressions of SOD isozyme in muscle, eyeball, hepatopancreas and heart were researched. The results showed that one band （Sod-1） expressed in muscle, eyeball and heart respectively, and two （Sod-1 and Sod-2） expressed in hepatopancreas. The Sod-1 in hepatopancreas expressed the thickest. Sod-2 was tested only in hepatopancreas. The expression of Sodl in muscle showed singleton. Expressions of SOD isozyme in different tissues appeared distinct tissue specificity.

关 键 词：克氏原螯虾 SOD同工酶 电泳 组织特异性 

分 类 号：Q55[生物学—生物化学]