tTG蛋白与子宫内膜癌细胞凋亡和紫杉醇化疗敏感性  

Tissue transglutaminase inhibition promotes cell death and chemosensitivity in ovarian cancer

作　　者：杨明芳[1] 李茗[1] 王泽华[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院妇产科,湖北武汉430022

出　　处：《中国妇幼保健》2008年第32期4614-4617,共4页Maternal and Child Health Care of China

摘　　要：目的:探讨组织转谷氨酰胺酶抑制剂(monodansylcadaverine,MDC)对体外培养的人子宫内膜癌Ishikawa细胞凋亡的诱导作用和对紫杉醇(TS)的化疗增敏性。方法:用MTT法检测逐渐递增的MDC药物浓度和不同作用时间对细胞增殖的影响。用流式细胞技术检测MDC对细胞凋亡和紫杉醇的化疗敏感性的影响。结果:Ishikawa细胞中,MDC处理组和对照组凋亡率差异有统计学意义(P<0.05);MDC+TS组的细胞凋亡率亦高于TS组(P<0.05);同时,MDC对细胞凋亡的诱导作用呈一定的剂量时间依赖关系。结论:组织转谷氨酰胺酶对维持子宫内膜癌细胞生长有一定作用,抑制酶的活性可以促进细胞凋亡并增加细胞对紫杉醇的化疗敏感性。Objective： To investigate whether tissue transglutaminase （tTG） inhibitor （ monodansylcadaverine, MDC ） promotes cell death and sensitivity of endometrial cancer cell fine Ishikawa to taxinol （TS） chemotherapy in vitro. Methods： Endometrial cancer cell line Ishikawa was selected. The expression of tTG was detected by immunofluorescenee technology and reverse transcription polymerase chain reaction （ RT - PCR）. The influence of gradually increased doses and time of MDC on survival in Ishikawa cell were detected by MrlT. The effect of MDC on apoptosis and sensitivity of Ishikawa cell to TS chemotherapy in vitro were measured by flow eytometry （FCM） . Results： Statistical analysis showed differences between the vehicle only - treated controls and the MDC - treated groups （ P 〈 0. 05）. Meanwhile, apoptotic percent of MDC ＋ TS groups were significandy higher （ P 〈 0. 05 ） compared to those of the TS groups. MDC - induced apoptosis in Ishikawa cell line depended on the time and doses of MDC used. Conclusion： tTG has some effect on growth of endometrial cancer cell and suppressing its activty can promote cell death and sensitivity of endometrial cancer cell to taxinol chemotherapy in vitro.

关 键 词：组织转谷氨酰胺酶 子宫内膜癌 紫杉醇 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]