柱前衍生化-气相色谱/质谱法测定细胞中的对-正壬基酚  

DETERMINATION OF NONYLPHENOL IN CELLS BY PRE-COLUMN DERIVATIZATION AND GAS CHROMATOGRAPHY COUPLED WITH MASS SPECTROMETRY

作　　者：高子燊[1] 鲜啟鸣[1] 韩晓冬[2] 潘旭萍[2] 冯建昉[1] 于红霞[1] 

机构地区：[1]南京大学环境学院,污染控制与资源化研究国家重点实验室,南京210093 [2]南京大学医学院免疫与生殖生物学实验室,南京210093

出　　处：《环境化学》2008年第6期819-822,共4页Environmental Chemistry

基　　金：国家自然科学基金国际(地区)合作与交流项目(No.20518002)资助

摘　　要：采用柱前衍生化-气相色谱/质谱法测定染毒细胞以及细胞质中对-正壬基酚(4-n-NP)的含量,并初步探讨了4-n-NP在细胞中的分布.在不同介质中加标回收率在76.5%—98.9%之间.利用本方法测定实际样品发现:在浓度为10μmol·l-1和20μmol.l-1的4-n-NP溶液中暴露12h,105个细胞中4-n-NP的含量分别为21.0ng和43.0ng,而相应的细胞质中4-n-NP的含量分别为14.2ng和28.4ng,显示有67.6%和66.0%的4-n-NP分布在细胞质中.In order to study the distribution of 4-n-NP in cells, a pre-eolumn derivatization method coupled with GC/ MS was set up to analyze 4-n-NP quantifieationally in cells and their cytoplasm. The spiked recoveries ranged from 76.5% to 98.9% for different mediums. After cells exposed to 10 μmol ·l^-1 and 20 μmol ·l^-1 4-u-NP solutions for 12 hours, the amount of 4-n-NP were 21.0ng and 43.0ng in 105cells and 14. 2ng and 28.4 ng in cytoplasm of 10^5 cells, respectively. The results indicate that about 67.6% and 66.0% of 4-n-NP were in cells cytoplasm.

关 键 词：对-正壬基酚 衍生化 气相色谱/质谱 细胞 细胞质 

分 类 号：X132[环境科学与工程—环境科学]