氟尿嘧啶致肠黏膜损伤修复过程中肠上皮干细胞的动态观察  

Dynamic observation of intestinal epithelial stem cells of small intestine during the injured- repaired progress induced by 5-FU

作　　者：吴承堂[1] 罗育其[1] 闻英[1] 

机构地区：[1]南方医科大学南方医院普通外科,广州510515

出　　处：《中华胃肠外科杂志》2008年第6期572-575,共4页Chinese Journal of Gastrointestinal Surgery

基　　金：广东省自然科学基金（032901）;广东省医学科研基金（A2004393）

摘　　要：目的观察氟尿嘧啶（5-FU）致肠黏膜损伤修复过程中肠上皮干细胞的动态变化。方法成年C57BL／6J小鼠50只，其中40只给予连续5d腹腔注射5-Fu（30mg·kg^-1·d^-1），另10只腹腔注射等量PBS作为空白对照。于处理后1、3、5和7d剖杀取出中段小肠，光镜下观察肠黏膜病理改变：流式细胞术检测肠黏膜细胞中Rhdamine123（Rho）染色阴性细胞比例变化情况：免疫组化分析Musashi-1fmsi-1）表达阳性细胞数量及其百分比。结果与对照组比较，5-Fu作用后小鼠肠黏膜萎缩，间质有少量炎性细胞浸润：Rho染色阴性细胞比例显著增高，以处理后1d最高，随后逐渐下降（P〈0．011；msi-1表达阳性细胞数量略有降低，但差异无统计学意义（p〉0．05）；msi．1表达阳性细胞百分比显著增高（P〈0．01）R与Rho染色阴性细胞比例呈显著正相关（P〈0．01，r=0．7551。结论Rho染色阴性细胞中含有丰富的肠上皮干细胞．msi-1表达阳性的肠上皮干细胞可能起着修复5-FU致肠黏膜损伤的作用。Objective To investigate the dynamic changes of intestinal epithelial stem cells during the injured-repaired progress induced b） 5-FU. Methods Fifty adult C57BL/6J mice were enrolled in this study, 40 of them were intraperitoneally injected with 5-FU （30 mg per kg of body weigh） for five days, and 10 of them intraperitonally injected with PBS as control. At day 1, 3, 5, 7 after treatment, the mice were killed and middle intestine was taken. Pathology was examined by HE staining. Musashi-1 （msi-1） expression was detected by immunohistoehemical technique. The percentage of Rho low staining ceils was detected by flow cytometry. Results After treatment with 5-FU, the intestinal mueosa was damaged. The Rho low staining cells were increasing, and at day 1 after treatment, the percentage of Rho low staining cells reached the highest level（P〈0.01 ）. The number of cells expressing msi-1 did not change significantly（P〉0.05）, but the percentage of positive msi-1 cells increased significantly （P〈0.01）. There was positive correlation between the percentage of Rhodmine 123 low staining cells and positive msi-I cells in each group （r=0.867, P〈O.01 ）. Conclusions The Rho low staining cells may contain rich intestinal epithelial stem cells. The intestinal epithelial stem cells expressing msi-1 can regenerate the damage of intestinal mucosa induced by 5-FU.

关 键 词：肠上皮干细胞 氟尿嘧啶 Rhdamine 123 MUSASHI-1 

分 类 号：R574[医药卫生—消化系统]