海螵蛸多糖对小鼠胃黏膜保护作用的研究  

Protective effect of Cuttlebone polysaccharides on gastric mucosa in mice

作　　者：郭一峰[1] 周文丽[1] 张建鹏[1] 刘军华[1] 冯伟华[1] 焦炳华[1] 

机构地区：[1]第二军医大学基础部生物化学与分子生物学教研室,上海200433

出　　处：《第二军医大学学报》2008年第11期1328-1332,共5页Academic Journal of Second Military Medical University

摘　　要：目的:用海螵蛸中提取的多糖盐洗组分(CBP-s)预处理小鼠后,用无水乙醇灌胃处理,观察小鼠胃黏膜的损伤程度,探究其保护胃黏膜的机制。方法:用CBP-s分别预处理小鼠3d和5d,再用无水乙醇诱导其胃黏膜损伤,测定溃疡指数、胃内容物pH值,以及胃黏膜的一氧化氮(NO)、谷胱甘肽(GSH)、丙二醛(MDA)和过氧化物歧化酶(SOD)等指标。结果:100、200和400mg/kg CBP-s预处理3 d和5 d的小鼠溃疡指数下降;100、200和400mg/kg CBP-s预处理3d和5d,胃内容物pH值分别是3.09±0.20、3.43±0.23、3.32±0.15、3.34±0.25、3.38±0.15、3.68±0.24;400mg/kg CBP-s预处理3d和5d,胃黏膜的MDA含量较造模组都有显著下降[(125.41±28.79)vs(145.80± 26.13);(153.88±21.19)vs(127.14±5.68)nmol/g,P<0.05];除了100mg/kg预处理3d组,各剂量组均能显著提高GSH和NO的含量[GSH:(282.93±32.33),(293.57±20.76),(260.79±39.23),(253.71±21.11),(359.38±10.89)mg/g,P<0.05];NO:(23.40±4.41),(25.83±4.89),(21.60±3.05),(22.75±2.44)μmol/g,P<0.05];各组的SOD活性均有显著上升[(30.61±6.37),(45.35±1.90),(47.12±4.68),(36.48±5.64),(41.38±8.23),(39.42±8.76)U/mg,P<0.05]。结论:CBP-s对乙醇诱导的小鼠胃黏膜具有细胞保护作用。Objective： To observe the protective effect of cuttlebone polysaccharides （CBP-s） on gastric mucosa lesions induced by absolute ethanol in mice,and to study the related mechanism. Methods：Mice were pretreated by CBP-s for 3 or 5 days and then were administered with absolute ethanol orally to induce gastric mucosa lesions. Then the ulcer index, pH value of gastric contents,the levels of nitric oxide （NO）,malondialdehyde （MDA）,reduced glutathione （GSH）, and the activity of SOD in the gastric mucosa were studied. Results： Mice gastric ulcer indices decreased after pretreated with 100 mg/kg, 200 mg/kg, and 400 mg/kg CBP-s for 3 or 5 days. The pH values of gastric contents after 3 day pretreatment were 3.09±0.20,3.32±0.15, 3.38 4±0.15, and after 5 day pretreatment were 3.43 ± 0.23,3.34 ± 0.25,3.68 ± 0.24, respectively. MDA contents of gastric mucosa after 3 day-pretreatment with 400 mg/kg CBP-s and after 5 day-pretreatment with alt 3 concentrations were （125.41± 28.79） ,（145.80±26.13）,（153.88±21.19）,and （127.14±5.68） nmol/g,respectively,all significantly lower than that of the model group （P〈0. 05）. GSH and NO contents were significantly increased in all but 100 mg/kg for 3 days group （GSH： [282. 93±32. 33],[293. 57±20. 76],[260. 79±39. 23],[253. 71±21. 11],[359.38±10.89] mg/g,P〈0.05）,（NO：[23.40± 4.41], [25. 83 ± 4. 89 ], [21. 60 ± 3. 05 ], and [22. 75 ±2. 44 ] μmol/g, P 〈 0. 05 ）. The activities of SOD were significantly increased in all groups （[30.61±6.37] ,[45.35±1.90],[47.12±4.68],[36.48±5.64] ,[41.38±8.23] ,and [39. 42±8. 76] U/mg,P〈0.05）. Conclusion： Our results indicate that CBP s can protect ethanol-induced gastric mucosa from injury in mice.

关 键 词：胃溃疡 胃黏膜 海螵蛸 多糖 溃疡指数 细胞保护 

分 类 号：R573.11[医药卫生—消化系统]