整合连接激酶在血管内皮生长因子诱导视网膜新生血管形成的信号传导通路中的作用  

Integrin-linked kinase and retinal neovascularization induced by vascular endotelial growth factor

作　　者：邹贺[1] 黎晓新[1] 于文贞[1] 闫征[2] 张同河[1] 张晓光[3] 

机构地区：[1]北京大学人民医院眼科中心,100730 [2]北京大学人民医院中心实验室,100730 [3]吉林大学第二医院眼科

出　　处：《中华眼底病杂志》2008年第6期431-435,共5页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的观察整合连接激酶（ILK）在血管内皮生长因子（VEGF）诱导的视网膜新生血管形成过程中的作用。方法在体外培养的视网膜脉络膜血管内皮细胞（RF／6A细胞系）中，用LY294002抑制ILK活性或siRNA转染敲减ILK表达后，检测ILK对VEGF诱导的细胞黏附、增生、迁移及内皮细胞管状结构形成的作用；并在动物模型水平观察LY294002抑制ILK活性后对视网膜新生血管形成的影响。结果正常对照组、VEGF处理组、LY294002抑制组、siRNA转染组细胞黏附实验结果分别为（0．0726±0．01961）、（0．1137±0．02631）、（0．0837±0．01503）、（0．0853±0．02454），VEGF处理组与正常对照组比较，差异有统计学意义（t=4．211，P〈0．01）；LY294002抑制组以及siRNA转染组与VEGF处理组相比，差异有统计学意义（t=3．074和2．91，P〈0．01）。正常对照组、VEGF处理组、LY294002抑制组细胞增生实验结果分别为（0．4162±0．1392）、（0．6412±0．2420）、（0．4476±0．1834），VEGF处理组较正常对照组比较，差异有统计学意义（t=2．608，P〈0．05）；LY294002抑制组与VEGF处理组相比，差异也有统计学意义（t=2．244，P〈0．05）。正常对照组、VEGF处理组、LY294002抑制组细胞迁移实验结果分别为（83．66±30．283）、（248±74．748）、（138．5±38．167），VEGF处理组与正常对照组比较，差异有统计学意义（t=5．436，P〈O．01）；LY294002抑制组与VEGF处理组相比，差异也具有统计学意义（t=3．682，P〈0．01）。血管内皮细胞管状结构形成实验显示，ILK活性或表达受到抑制后无明显管状结构形成。动物实验显示腹膜下注射LY294002使视网膜后极部无灌注区面积从（62798±16995．62）μm^2增加至（84722．65±10435．01）μm^2，二者比较，差异有统计学意义（t=3．476，P〈0．01）。结论应用LY294002抑制ILK活性或siRNA转染敲减ILK表达使细�Objective To evaluate the effect of integrin-linked kinase （ILK） in the process of retinal neovascularization induced by vascular endothelial growth factor （VEGF）. Methods The ILK activities of retinal choriodal endothelial cell line RF/6A were inhibited by LY294002 or siRNA knockdown. VEGF-induced changes of cell adhesion, proliferation, migration and endothelial cell tubeformation were measured then. The in-vivo effects of ILK were also assessed by intraperitoneal injection of LY294002 into an animal model of RNV. Results The cell adhesion measurements of control group, VEGF group, VEGF＋LY294002 group and VEGF＋siRNA group were 0. 0726±0. 019 61, 0. 1137± 0.026 31, 0.0837± 0.015 03 and 0.0853±0.024 54, respectively. The difference was statistically significant between VEGF group and control group （t = 4. 211, P〈 0.01）, and between （VEGF ＋LY294002） group or （VEGF--siRNA） group and control group （t= 3. 074, 2.91,P〈0.01）. The cell proliferation results of control group, VEGF group and VEGF＋LY294002 group were 0. 4162±0. 1392, 0. 6412 ± 0. 2420, 0. 4476 ±0. 1834 , respectively. The difference was statistically significant between VEGF group and control group（t=2. 608,P〈0.05）, and between （VEGF＋LY294002） group and VEGF group （t = 2. 244,P〈0.05）. The cell migration results of control group, VEGF group and VEGF-a LY294002 group were 83.66±30. 283, 248±74. 748, 138.5±38. 167, respectively. The difference was statistically significant between VEGF group and control group （t = 5. 436, P〈0. 01）, and between （VEGF＋LY294002） group and VEGF group （t= 3. 682,P〈0.01）. There was no obvious tube-formation after ILK activity was inhibited or knocked down. The non-perfusion areas were increased from （62 798± 16 995.62）μm^2 to （84 722. 65±10 435.01）μm^2 after intraperitoneal injection of LY294002 into animal model of RNV, the difference was statistically significant （t = 3. 476,P〈0.01）. Conclusions ILK may play an important role in th

关 键 词：血管内皮生长因子类 视网膜新生血管化 整合素类 信号传导 动物实验 

分 类 号：R774.1[医药卫生—眼科]