口腔鳞癌患者淋巴细胞对人疱疹病毒6型增殖应答受损  

Impairment of lymphoproliferative response to human herpesvirus-6 in oral squamous cell carcinoma patients

作　　者：王芳[1] 姚堃[2] 周锋[2] 杨婕[3] 

机构地区：[1]南京医科大学第一附属医院(江苏省人民医院)临床检验中心 [2]南京医科大学微生物学与免疫学系 [3]南京医科大学口腔医学院,江苏南京21002

出　　处：《基础医学与临床》2008年第11期1134-1137,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(30771961)

摘　　要：目的研究口腔鳞癌患者淋巴细胞对人疱疹病毒6型(HHV-6)特异性增殖应答,初步探讨HHV-6在口腔鳞癌发病机制中的作用。方法间接免疫荧光法(IIF)检测血浆中抗HHV-6IgG;免疫微磁珠分离CD4+T及CD8+T细胞;3H-TdR法检测CD4+T和CD8+T细胞及PBMCs的增殖水平;FACS分析CD4+C25+调节性T细胞(Treg)的比例。结果口腔鳞癌组血浆中抗HHV-6IgG阳性数为8/8,正常对照组为12例(12/20);口腔鳞癌组PBMCs及CD4+T细胞对HHV-6的增殖水平显著低于HHV-6潜伏感染组与未感染组(P<0.05);HHV-6潜伏感染组PBMCs及CD4+T细胞对HHV-6的增殖水平显著低于未感染组(P<0.05);口腔鳞癌组外周血中CD4+C25+Treg比例明显高于HHV-6潜伏感染组与未感染组(P<0.05)。结论口腔鳞癌患者的HHV-6特异性CD4+T细胞增殖应答减弱,可能在口腔鳞癌的发生发展中起一定作用。Objective To study the lymphoproliferative responses to HHV-6 （Human Herpesvirus-6, HHV-6 ） among patients with oral squamous cell carcinoma, and to discuss the role of HHV-6 in the pathogenesis of oral squamous cell carcinoma. Methods IgG antibody to HHV-6 in plasma was identified by indirect immunofluorescence （IIF）. Immuno-magnetic beads were used to prepare CD4^＋ T ceils and CD8^＋ T cells. The proliferation of CD4^＋ T ceils, CD8^＋ T cells and PBMCs stimulated with HHV-6 or anti-CD3 antibodies was measured by 3H-thy- midine uptake. The percentage of CD4^＋ C25^＋ Treg cells （Regulatory T cell, Treg）within the peripheral blood CD4^＋ T cell compartment was analyzed by flow cytometry. Results Significantly higher proportion of patients with oral squamous cell carcinoma had IgG antibody to HHV-6 （8/8） in plasma as compared with those in control subjects （ 1 2 / 2 0 ） ; The proliferative responses of PBMCs and CD 4^＋ T cells from patients with oral squamous cell carcinoma to HHV-6 were significantly decreased as compared to those from HHV-6-infected and healthy individuals （P 〈 0. 05;P 〈 0. 05 ）. The proliferative responses of PBMCs and CD4^＋ T cells from HHV-6-infected healthy individuals to HHV-6 were significantly decreased as compared to those from HHV-6-megative healthy individuals （P 〈 0. 05 ） ; Patients with oral squamous cell carcinoma had a significantly higher percentage of CD4^＋ C25^＋ Treg cells in peripheral blood CD4^＋ T cell compartment as compared with HHV-6 infected and healthy individuals did. Conclusion These results demonstrated that impairment of HHV-6-specific CD4^＋ T cell immune responses in patients with oral squamous cell carcinoma, therefore, HHV-6 potentially plays a role in the pathogenesis of oral squamous cell carcinoma.

关 键 词：人疱疹病毒6型 口腔鳞癌 淋巴细胞增殖 CD4^+C25^+调节性T细胞 

分 类 号：R739.8[医药卫生—肿瘤]