Nestin在食管癌变过程中的表达及意义  

The Expressions and Significance of Nestin in Various Esophageal Lesions

作　　者：苏蔚[1] 房成[2] 张高磊[1] 崔静[1] 王永霞[1] 焦云娟[1] 赵卫星[1] 

机构地区：[1]新乡医学院病理教研室,河南新乡453003 [2]新乡医学院第三附属医院,河南新乡453003

出　　处：《肿瘤基础与临床》2008年第6期461-463,共3页journal of basic and clinical oncology

摘　　要：目的通过检测巢蛋白(Nestin)在食管正常上皮及不同病变组织中的表达及与血管内皮生长因子(VEGF)的关系,探讨巢蛋白在食管癌的发生、浸润和转移中的作用。方法正常食管断端组织40例、非典型增生42例、原位癌30例、食管浸润癌127例,免疫组织化学及实时定量RT-PCR检测Nestin的表达并分析其与VEGF的协同关系。结果Nestin的阳性表达率随病变的进展逐步增高,转移组的阳性表达率(96.61%)显著高于未转移组(89.66%)(P<0.05),而转移组内不同分化程度间的表达率无显著差别;Nestin与VEGF在浸润癌组织中的表达具有显著相关性(2χ=8.326,P<0.05,c=0.2578)。结论Nestin表达提示食管上皮发生了癌变以及癌细胞获得了远处转移的能力,食管鳞癌去分化及浸润、转移能力的增强与Nestin及VEGF高表达有关。Objective To explore the role of Nestin in the carcinogenesis, infiltration and metastasis of esophageal carcinomas by detecting the expressions of Nestin in the normal esophageal epithelium and various esophageal lesions and the correlation with VEGF. Methods The expressions of Nestin and its relationship with VEGF were detected by immunohistochemistry method and RT-PCR in 40 normal esophageal incisal margin tissues, 42 atypical hyperplasia, 30 carcinomas in situ, 127 esophageal invasive carcinomas. Results The positive ratio of Nestin was accordingly increased with the lesions progress. The positive ratio in the metastasis group （96.61%） was significantly higher than that in the non-metastasis group（ 89.66% ） （P 〈 0.05 ） ; but there was no significant difference between the degrees of differentiation （P 〉 0.05 ）. There was significant correlation between the expressions of Nestin and that of VEGF in the infiltrating carcinomas（χ^2 = 8. 326, P 〈 0.05, c =0. 2578）. Conclusion The expressions of Nestin seemed to indicate the canceration of esophageal epithelium and the malignant cells obtained the ability of metastasis. The excessive expressions of Nestin and VEGF were correlated with the dedifferentiation, powerful invasion and metastasis of esophageal squamous carcinomas.

关 键 词：食管癌 巢蛋白 血管内皮生长因子 

分 类 号：R735.1[医药卫生—肿瘤]