脑胶质瘤SHG-44细胞株照射后子代辐射敏感性  

Radiosensitivity of filial generation from irradiated human glioma cell line SHG-44

作　　者：李超[1] 李莉[2] 许昌韶[1] 周菊英[2] 徐晓婷[2] 俞志英[2] 

机构地区：[1]上海交通大学医学院苏州九龙医院放疗科,215021 [2]苏州大学附属第一医院放疗科,215006

出　　处：《国际放射医学核医学杂志》2008年第4期236-239,共4页International Journal of Radiation Medicine and Nuclear Medicine

摘　　要：目的探讨脑胶质瘤SHG-44细胞株照射子代生长特性及辐射敏感性变化。方法培养人脑胶质瘤SHG-44细胞株照射后的存活子代细胞，测定其群体倍增时间，并进行集落形成实验和流式细胞仪检测，分析其辐射敏感性和细胞周期变化。结果SHG．44细胞群体倍增时间为（22．78±2．61）h，SHG-44细胞经6MVX射线10Gv照射后存活子代SHG-44—10细胞群体倍增时间为（30．46±2．73）h（F=7．878，P〈0．05）。SHG-44细胞和SHG-44-10细胞再次2Gy照射后的存活分数分别为70．8％、80．6％。与SHG-44细胞相比，SHG-44—10细胞在GJM期比例减少，S期比例增多。结论SHG-44细胞照射后存活子代细胞增殖延缓，辐射敏感性下降。Objective To investigate the radiosensitivity and growth features of the progeny of irradiated human glioma cell line SHG-44. Methods The SHG-44 cells were irradiated with 6 MV X ray and the progeny of the ceils were cultured. The population doubling time （PDT） was detected pre-and postirradiation. The radiosensitivity of the progeny of irradiated human glioma cell line SHG-44 was measured by clone forming assay. The cell cycle distribution was analysed by flow cytometry. Results The PDT of SHG- 44 cells before irradiation was （22.78±2.61）h. The PDT of irradiated SHG-44 ceils at dose of 10 Gy were （30.46±2.73） h （F=7.878, P〈0.05）. The surviving fraction of SHG-44 cell was 70.8%, SHG-44-10 cell was 80.6%. SHG-44 cells showed significant G2/M phase decrease and S phase arrest after irradiation. Conclusion Growth delay and declined radiosensitivity are confirmed in the progeny of irradiated SHG-44 cells.

关 键 词：神经胶质瘤 肿瘤细胞 培养的 辐射耐受性 细胞周期 

分 类 号：R73[医药卫生—肿瘤]