肺鳞癌及癌前病变变异型CD_(44)和增殖细胞核抗原的表达  

Expression of CD_(44) and proliferating cell nuclear antigen (PCNA) in squamous cell carcinomas of lung and precancerous lesions

作　　者：姜彦多[1] 王继红[1] 王晓牧[1] 何安光[2] 常青[1] 聂明豪 王婷婷[1] 

机构地区：[1]解放军第202医院病理科,沈阳110003 [2]中国医科大学肿瘤研究所

出　　处：《诊断病理学杂志》1997年第4期214-216,共3页Chinese Journal of Diagnostic Pathology

摘　　要：应用免疫组化方法在含有肺鳞癌及其癌前病变的标本上观察了变异型CD44（CD44v）和增殖细胞核抗原（PC－NA）在肺鳞癌发生过程中出现表达的时间和频率。结果癌膏正常支气管上皮细胞未见CD44v表达，在鳞状化生的支气管上皮细胞、轻→中→重度不典型增生、原位癌、浸润癌，CD44v表达的阳性率逐渐升高，阳性细胞也逐渐增多。表达CD44v的不典型增生的支气管上皮，其相应的鳞癌CD44v也呈阳性表达。PCNA的表达也呈现上述特点，但出现表达的时间早于CD44v。结果表明，变异型CD44和PCNA可作为判断肿瘤发生可能性的有用的标记物，在肺鳞癌的早期诊断中具有重要意义。Immunohistochemical analyses for CD44v and PCNA expression in specimens of squamous cell carcinomas of lung with adjacent normal epithelium and/or precancerous lesions from 34 patients were made. Mernbrane ex-pression of CD44v. was present in 0 of 12 specimens with normal mucosa, in 2 of 14 with squamous metaplasia, in 3 of 13 with mild dysplasia, in 5 of 17 with moderate dysplasia, in 12 of 21 with severe dysplasia, in 5 of 9 with car-cinoma in situ, and in 21 of 34 with invasive tumor, while PCNA labeling index was 6. 5± 3. 3, 9. 4± 4. 7, 12. 5±8. 7, 43. 3 ± 14. 9, 68. 1± 9. 1, 74. 6 ± 8. 2, 650 4± 23. 6 respectively in the above mentioned lesions. With the progress of bronchial lesions from mild dysplasia to severe dysplasia to carcinoma in situ and invasive carcinoma,the immunostained cells of CD44v and PCNA increased in number and appeared in the upper cell layers of the epithe-lium. These findings indicate that CD44v expression is detectable in precancerous bronchial lesions and suggest that routine assessment of CD44± and PCNA expression be valuable for the early diagnosis of squamous cell lung carcino-ma.

关 键 词：肺肿瘤 癌前病变 CD_44 增殖细胞核抗原 免疫组织化学 

分 类 号：R734.202[医药卫生—肿瘤]