溶菌酶与α-MnO_2间的界面吸附作用  

The adsorption of lysozyme on the surface of α-MnO_2

作　　者：蔡爱军[1,2] 马子川[1,3] 刘敬泽[1] 

机构地区：[1]河北师范大学生命科学学院,石家庄050016 [2]河北科技师范学院生命科学系,秦皇岛066000 [3]河北师范大学化学与材料科学学院,石家庄050016

出　　处：《生态学报》2008年第11期5684-5690,共7页Acta Ecologica Sinica

基　　金：中国博士后科学基金资助项目(2005037630)~~

摘　　要：研究了溶菌酶(LSZ)与α-MnO2的界面吸附作用及其影响因素。结果表明,体系pH值是影响LSZ在α-MnO2表面吸附、脱附的重要因素。在pH3.8～10.8范围内,LSZ的吸附率随着体系pH值的升高而大致呈上升趋势。在较低pH值范围内(pH<7.1),LSZ的脱附率随pH的升高增幅较大;在较高pH值范围内(pH>7.1),LSZ的脱附率则随pH的升高而略微下降。但是,LSZ自α-MnO2表面的脱附率在各pH值下均处于较低水平,吸附反应具有较高的不可逆性。LSZ在α-MnO2表面的吸附率随离子强度的增大而增大,然而Na+、Ca2+和Mg2+3种离子对LSZ吸附率的影响没有差别。LSZ的等温吸附曲线符合Langmuir吸附方程式,形状呈L型。FTIR进一步证明,α-MnO2表面的确有LSZ分子吸附;吸附态LSZ分子的空间构象发生了改变。In this work, the adsorption of lysozyme（LSZ）on the α-MnO2and by which factors this process is affected were investigated. The results showed that the system pH value was a most important factor affecting adsorption and desorption of LSZ. In the pH range of 3.8 to 10.8, the adsorption percentage of LSZ on α-MnO2 was increased as pH value raised. The desorption percentage of lysozyme usually increased at low pH condition （ pH 〈 7. 1 ） but slightly decreased at high pH condition（ pH 〉 7. 1 ）. However, the desorption percentage was generally low at all pH values tested, indicating the irreversibility of protein adsorption to the surface of α-MnO2. We also found increasing the ionic strength might increase the LSZ adsorption percentage, but there was no difference on effects of Na^＋, Ca^2＋ and Mg^2＋ on this adsorption. The LSZ adsorption isotherm belonged to the L type, and was fitted well by the Langmuir model. FTIR spectra further approved that LSZ was attached to the surface of mineral, and the spacial structure of attached protein was changed.

关 键 词：LSZ α-MnO2 吸附 脱附 

分 类 号：X171[环境科学与工程—环境科学]