低频重复经颅磁刺激预处理对毛果芸香碱致痫大鼠海马GAD65、NMDAR1表达的影响  

The Effects of Pretreatment with Low-Frequency Repetitive Transcranial mMagnetic Stimulation on Expressions of Hippocampus GAD65 and NMDAR1 in Rats with Pilocarpine-Induced Seizures

作　　者：张军强[1,2] 余巨明[2] 王晓明[2] 赵红宁[1,2] 黄敏[2] 张小东[2] 赵晓琼[2] 黄慧[2] 胡建秀[2] 

机构地区：[1]成都中医药大学研究生院,四川成都610075 [2]川北医学院附属医院神经疾病研究所,四川南充637000

出　　处：《中国神经免疫学和神经病学杂志》2008年第6期430-433,475,共5页Chinese Journal of Neuroimmunology and Neurology

基　　金：四川省重点科技基金资助项目(05SCT022-016);四川省青年基金资助项目(04ZQ026-010)

摘　　要：目的探讨低频重复经颅磁刺激(rTMS)对毛果芸香碱致痫作用的影响及其机制。方法将大鼠随机分为rTMS刺激组、假刺激组和对照组,分别进行连续2周的相应预处理后,制作氯化锂-毛果芸香碱急性癫痫(EP)模型,观察造模后90min内痫性发作潜伏期和痫性发作行为表现。应用免疫组化方法观察各组大鼠海马区谷氨酸脱羧酶65(GAD65)、N-甲基-D-天门冬氨酸受体1(NMDAR1)的表达。结果rTMS刺激组大鼠痫性发作潜伏期较假刺激组和对照组延长,发作程度减轻(均P<0.05);rTMS刺激组大鼠海马区神经元GAD65的表达量明显高于对照组,NMDAR1表达量明显低于对照组(均P<0.05),但假刺激组与对照组二者表达差异无统计学意义(P>0.05)。结论低频rTMS预处理有明显抗痫作用,可能通过调节海马区GAD65和NMDAR1表达而起作用。Objective To study the effects of repetitive transcranial magnetic stimulation （rTMS） on Lithium Pilocarpine induced seizures and its possible mechanisms. Methods Rats were randomly divided into the 0.5 rTMS group,sham stimulation group and control group. After every day for 2 weeks＇ consecutive corresponding stimulations, the acute epilepsy model was made by Lithium Pilocarpine, and subsequently the latencies of seizures were observed, and the severity of seizure was quantitatively evaluated within 90 minutes after an intraperitoneal injection of Pilocarpine. Immunohistochemistry was used to observe the expressions of glutamic acid decar-boxylase65（GAD65） and N-methyl-D-aspartateR1 （NMDAR1） in the rat hippocampus of all studied groups. Results Compared with sham stimulation group and control group, the latency of seizure became much longer,and the severity of seizure was much milder（P〈0.05 for both） in rTMS group;rTMS group had increasedexpression of GAD65, but decreased expression of NMDAR1 （P〈0.05 for both） compared with the control group. There was no significant difference for expression of GAD65 and NMDAR1 between the sham stimulation group and control group（P〉0.05）. Conclusions The rats pretreated with 0.5 Hz rTMS showed clear resistance to pilocarpine-induced seizure, and this effect might he produced by regulating the expression of GAD65 and NMDAR1 in the hippocampus.

关 键 词：经颅磁刺激 癫痫 痫性发作 海马 谷氨酸脱羧酶 N-甲基-D-天门冬氨酸 

分 类 号：R744.5[医药卫生—神经病学与精神病学]