As_2O_3与Aspirin联合应用对人胃癌SGC-7901细胞凋亡的影响  

As_2O_3 and Aspirin induce apoptosis of human gastric cancer cells SGC-7901

作　　者：巩玉森[1] 仇玮[1] 刘霞[1] 吴永平[1] 李琳琳[1] 柳红[1] 

机构地区：[1]徐州医学院病理学教研室,江苏省徐州市221002

出　　处：《世界华人消化杂志》2008年第32期3594-3598,共5页World Chinese Journal of Digestology

基　　金：江苏省教育厅高校自然科学基金资助项目;No.06KJB310118~~

摘　　要：目的:观察As2O3与Aspirin联合应用对人胃癌SGC-7901细胞凋亡的影响并探讨其可能机制.方法:As2O3和Aspirin处理SGC-7901细胞,实验分为:阴性对照组、2mmol/L Aspirin组、1mmol/L Aspirin组、4μmol/L As2O3组、2μmol/L As2O3组和2μmol/L As2O3组+1mmol/L Aspirin组,流式细胞术检测As2O3和Aspirin单独及联合应用对SGC-7901细胞凋亡的作用,免疫细胞化学法检测Bcl-2和Bax蛋白的表达.结果:2μmol/L As2O3+1mmol/L Aspirin联合应用组与4μmol/L As2O3组、2mmol/L Aspirin组SGC-7901细胞在细胞周期G1期前均出现明显的亚二倍体凋亡峰,差异无明显统计学意义,与阴性对照组细胞、2μmol/L As2O3及1mmol/L Aspirin单药组相比,差异均有统计学意义(均P<0.05).As2O3与Aspirin联合应用组使Bcl-2表达下降,Bax表达增高,与阴性对照组、2μmol/L As2O3及1mmol/L Aspirin单药组相比,差异均具统计学意义(50.21%±5.94%vs91.65%±11.51%,88.66%±10.53%89.27%±9.84%;40.72%±9.54%vs21.03%±4.32%,23.07%±6.23%,22.67%±3.16%,均P<0.05).结论:As2O3和Aspirin可能通过改变Bcl-2和Bax表达诱导SGC-7901细胞凋亡,两者联合应用具有协同作用.AIM： To investigate the effect of As2O3 and Aspirin on apoptosis of human gastric carcinoma cells SGC-7901 and to explore its possible mechanism. METHODS： SGC-7901 cells were incubated in different concentrations of drugs, and then were divided into six groups： control group, Aspirin （2 mmol/L） group, Aspirin （1 mrnol/L） group, As2O3 （4 μmol/L） group, As2O3 （2 μmol/ L） group, and As2O3 （2 μmol/L） ± Aspirin （1 mmol/L） group. 72 hours after the treatment, apoptosis rates in each group were analyzed using flow cytometry. The expressions of Bcl-2 and Bax protein were measured by immunocytochemistry assay. RESULTS： Flow cytometry analysis revealed statistically significant difference between 2 μmol/L As2O3 ± 1 mmol/L Aspirin group and control group, 1 mmol/L Aspirin group, 2 μmol/L AS2O3 group （P 〈 0.05）, while there was no statistically significant difference between 2μmol/L As2O3 ± 1 mmol/L Aspirin group and 4 μmol/L As2O3 group, 2 mmol/L Aspirin group. Immunocytochemistry showed that there was down-regulated expression of Bcl-2 protein and up-regulated expression of Bax protein in 2 μmol/L As2O3 ± 1 mmol/L Aspirin group. Statistically significant difference was observed between 2μmmol/L As2O3 ± 1 mmol/L Aspirin group and control group, 1 mmol/L Aspirin group, 2 μmol/L As2O3 group （50.21% ± 5.94% vs 91.65% ± 11.51%, 88.66% ± 10.53%, 89.27% ± 9.84%; 40.72% ± 9.54% vs 21.03% ± 4.32%, 23.07% ± 6.23%, 22.67% ± 3.16%, all P 〈 0.05）. CONCLUSION： AS2O3 and Aspirin induce apoptosis of gastric cancer cells SGC-7901 possibly through suppressing Bcl-2 protein or enhancing Bax protein. Combination of As2O3 and Aspirin produces obvious synergistic effect.

关 键 词：三氧化二砷 阿司匹林 联合应用 胃癌细胞SGC-7901 凋亡 

分 类 号：R735.2[医药卫生—肿瘤] R973.2[医药卫生—临床医学]