Ki-67和VEGF在食管腺癌中的表达及意义  

Roles of Ki-67 and vascular endothelial growth factor in development of esophageal adenocarcinoma

作　　者：卢明芳[1] 王蓉[2] 

机构地区：[1]中国人民解放军南京军区福州总医院干部病房二科,福建省福州市350025 [2]中国人民解放军南京军区福州总医院消化内科,福建省福州市350025

出　　处：《世界华人消化杂志》2008年第32期3621-3625,共5页World Chinese Journal of Digestology

摘　　要：目的:通过对人食管腺癌(esophageal adenocarcinoma,EA)标本进行Ki-67和血管内皮生长因子(vascular endothelial growth factor,VEGF)的检测,揭示上述两者在EA发生发展中的作用及对预后的影响.方法:经病理确诊的EA患者50例,取其术后切除标本作为EA组,另取正常食管鳞状上皮50例作为正常对照组.采用免疫组化染色法检测EA标本中Ki-67蛋白和VEGF蛋白的表达情况,并进行组间比较和相关性分析.结果:Ki-67蛋白在正常鳞状上皮中几乎不表达,而在EA中呈中强阳性表达,广泛表达于腺癌细胞胞核,与正常食管鳞状上皮比较,差异均有显著性(χ2=85.463,P<0.01),且癌细胞分化程度和肿瘤病理学分期与Ki-67蛋白表达相关(χ2=13.11,χ2=17.78,均P<0.01);VEGF蛋白在正常鳞状上皮中不表达,在EA细胞胞质中呈强表达,与正常食管比较,差异均有显著性(χ2=80.60,P<0.01).且癌细胞分化程度、肿瘤的浸润程度及肿瘤病理学分期与VEGF蛋白表达相关(χ2=16.378,χ2=15.50,χ2=15.882,均P<0.05).Ki-67和VEGF在EA中表达升高,呈正协同关系(χ2=74.678,P=0.00).结论:Ki-67和VEGF在EA中表达均升高,说明两者的表达上调在EA的增殖及转移中起了重要作用,对肿瘤的预后起较好的高效能预测作用.AIM： To detect the expression of Ki-67 and vascular endothelial growth factor （VEGF） in esophageal adenocarcinoma （EA）, and to reveal their roles in EA occurrence and development as well as their influences on EA prognosis. METHODS： The expression levels of Ki-67 and VEGF protein were investigated in normal esophageal squamous epithelium （n = 50） and EA specimens （n = 50） using immunohisto-chemical staining respectively. The differences between groups was compared and correlation analysis was performed. RESULTS： Ki-67 was hardly expressed in normal esophageal squamous epithelium, but was strongly expressed in the cell nucleus of EA specimens. There was a significant difference in Ki-67 staining （χ^2= 85.463, P 〈 0.01） between them. Moreover, there were significant correlations between the expression of Ki-67 and cell differentiation or pathologic stages of EA （χ^2 = 13.11, 17.78, both P 〈 0.01）. Similarly, there was a significant difference in VEGF staining between normal esophageal squamous epithelium and EA specimens （χ^2 = 80.60, P 〈 0.01）, and VEGF expression was correlated with tumor differentiation degree, invasion depth and pathologic stages （2 = 16.378, 15.50, 15.882; all P 〈 0.05）; VEGF was strongly expressed in the cytoplasm of EA specimens. Ki-67 and VEGF expression were up-regulated synergically in EA （χ^2=74.678, P = 0.00）. CONCLUSION： Up-regulation of Ki-67 and VEGF may play an important role in the development of in EA progression.

关 键 词：食管腺癌 KI-67 血管内皮生长因子 免疫组化 

分 类 号：R735.1[医药卫生—肿瘤] R734.2[医药卫生—临床医学]