醇化烟叶表面可培养微生物的鉴定和系统发育分析  

Identification and Phylogenesis Analysis of Cultivable Microorganisms on Tobacco Leaf Surface During Aging

作　　者：杨金奎[1] 段焰青 陈春梅[1] 李庆华 黄静文[1] 张克勤[1] 

机构地区：[1]云南大学生物资源保护与利用重点实验室,昆明市650091 [2]红云烟草(集团)有限责任公司技术中心,昆明市650202

出　　处：《烟草科技》2008年第11期51-55,共5页Tobacco Science & Technology

基　　金：云南省科技攻关及高新技术发展计划“生化技术在构建中式卷烟中的应用”(2006GG22)

摘　　要：以K326烤烟品种为材料,对不同醇化时间烟叶表面的可培养微生物进行了分离,并通过分子生物学方法对这些微生物的种、属分类地位和系统发育关系进行了分析。结果表明:醇化烟叶表面可培养微生物的数量和种类随着醇化时间的延长呈明显的下降趋势;醇化烟叶表面的细菌主要包括芽孢杆菌和肠杆菌两个类群,其中,芽孢杆菌属的细菌为优势微生物类群。此外,醇化烟叶表面细菌的数量和烟叶部位等级也存在一定的相关性,即中部烟叶样品中细菌的数量高于下部烟叶样品,而上部烟叶样品中的细菌数量最少。而真菌的数量和种类与醇化时间和烟叶样品部位等级之间没有明显的相关性。The cultivable microorganisms were isolated from the leaf surface of flue-cured tobacco cv. K326 sampled at different aging stages, and the systematic status of these microorganisms and their phylogenetic relationship were analyzed with molecular biological method. The results showed that the amount and races of cultivable microorganisms on leaf surface obviously decreased with the prolongation of aging time. The major bacteria are Bacillus sp. and Enterobacter sp. ; and Bacillus sp. is the dominant. Moreover, there is certain relativity between the amount of bacteria and the stalk position of tobacco leaf, namely, the amount of bacteria on middle leaf is larger than that on lower leaf, and that on upper leaf is the smallest. However, there is no obvious relativity between the amount and race of fungi and the length of aging time or stalk position of tobacco leaf.

关 键 词：烤烟 醇化 微生物 系统发育 

分 类 号：TS444[农业科学—烟草工业] TS414