克氏原螯虾不同发育阶段卵巢中EST同工酶比较  

Comparison of EST Isozyme in the Ovary of Procambarus clarkii in Different Developmental Stages

作　　者：崔雪[1,2] 张引[2] 黄孝湘[2] 徐东生[2] 吕邦玉[2] 

机构地区：[1]华中农业大学水产学院,湖北武汉430074 [2]孝感学院生命科学技术学院,湖北孝感432100

出　　处：《安徽农业科学》2008年第31期13666-13667,共2页Journal of Anhui Agricultural Sciences

基　　金：孝感学院校内项目

摘　　要：[目的]研究克氏原螯虾(Procambarus clarkii)卵巢成熟过程EST的变化。[方法]采用聚丙烯酰胺凝胶电泳方法,分析了卵黄形成期和成熟期卵巢中酯酶同工酶(EST)的表达情况。[结果]所研究的两个阶段卵巢中EST同工酶的表达可分为Ⅰ、Ⅱ、Ⅲ共3个区。其中Ⅰ区和Ⅱ区卵黄形成期EST表达较强,酶带明显比成熟期浓,尤其是Ⅰ区,卵黄形成期EST同工酶的表达最强。相反,在Ⅲ区,成熟期EST酶带明显比卵黄形成区浓。[结论]在克氏原螯虾卵子成熟过程中,EST基因的表达存在时间差异性。[Objective] The purpose was to study the changes of EST in the ovary of Procambarus clarkii in the mature period.[Method] By using the method of polyacrylamide gel electrophoresis,the expression of esterase （EST） isozyme in the ovary in vitellogenesis stage and mature stage was analyzed.[Result] The expression of esterase isozyme in the ovary in two stages could be divided into 3 regions（Ⅰ,Ⅱ and Ⅲ）.In the vitellogenesis period,EST in region Ⅰ and Ⅱ expressed more strongly and the isozyme bands were obviously dense than that in mature stage.Especially,the expression of EST isozymes in region Ⅰ in the vitellogenesis period was strongest.On the contrary,EST isozyme bands in region Ⅲ in the mature stage were obviously dense than that in the vitellogenesis region.[Conclusion] The expression of EST gene had temporal difference in the mature stage of P.clarkii ovary.

关 键 词：克氏原螯虾 EST同工酶 卵巢 卵黄形成期 成熟期 

分 类 号：S966.12[农业科学—水产养殖]