皮肤T细胞淋巴瘤皮损与外周血中TCRγ基因重排的检测  

Primary research on TCRγ gene rearrangements in lesions and PBMC from the patients with cutaneous T cell lymphoma

作　　者：薛峰[1] 沈小雁[1] 施若非[1] 李霞[1] 陈小英[1] 郑捷[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院皮肤科,瑞金-耶鲁皮肤淋巴瘤治疗中心,上海200025

出　　处：《临床皮肤科杂志》2008年第12期766-768,共3页Journal of Clinical Dermatology

基　　金：上海交通大学医学院科研基金(06XJ21023)

摘　　要：目的:了解已确诊为皮肤T细胞淋巴瘤(CTCL)患者的皮损和外周血中T细胞受体γ链基因重排(TCRγGR)情况。方法:利用PCR测定21例CTCL患者皮损及外周血TCRγGR,扩增产物经琼脂糖及变性梯度凝胶电泳检测。结果:CTCL患者皮损中TCRγGR阳性率显著高于外周血(P<0.05);病程≥12个月的患者外周血中TCRγGR阳性率显著高于病程<12个月的患者(P<0.05);年龄≥60岁患者皮损和外周血中TCRγGR阳性率显著高于<60岁患者(P<0.05)。结论:在CTCL患者中存在TCRγGR,早期出现在皮损中的TCRγGR可作为CTCL辅助诊断,并有益于CTCL的早期诊断,外周血中检测出TCRγGR可作为皮损阳性结果的补充。Objective： To analyze the TCRγ gene rearrangements （TCRγ GR） from the skin specimens and peripheral blood mononuclears cells （PBMC） of patients who have been diagnosed as cutaneous T cell lymphoma（CTCL）. Methods： Twenty-one CTCL patients were identified by means of PCR and products were analyed by agarose electrophoresis and denaturing gradient gel electrophoresis （DGGE） procedure. Results： TCRγGR positivity of samples from CTCL patients lesions was statistically significant as compared with that from the PBMC source （P〈 0.05）. The positivity rate of recombination was higher in samples from patients with more than 12 month course of diseases than from whom with less than 12 months （P〈0.05）. The common thing from these 2 groups was that, the positive TCRγGR rate was higher in patients aged over 60 years than patients aged younger than 60 （P 〈 0.05）. Conclusions： In CTCL patients, there is TCRγ gene rearrangement. The TCRγGR detection in the skin lesions is useful to early diagnosis of CTCL, while detecting TCRγ GR in PBMC could be supplement to it.

关 键 词：淋巴瘤 T细胞 皮肤 受体 T细胞 γ链基因重排 

分 类 号：R730.263[医药卫生—肿瘤]