向日葵BADH基因部分序列的克隆与分析  

Cloning and Analysis of BADH Gene from Helianthus annuus

作　　者：易乐飞[1] 王萍[1] 周向红[1] 

机构地区：[1]淮海工学院海洋学院,江苏连云港222005

出　　处：《安徽农业科学》2008年第32期14003-14004,共2页Journal of Anhui Agricultural Sciences

基　　金：淮海工学院自然科学基金(Z2007035);淮海工学院引进人才科研启动基金(KK01073)资助

摘　　要：[目的]为进一步利用向日葵BADH基因提供理论和实践依据。[方法]以电子克隆技术为基础,通过RT-PCR获取向日葵BADH基因的部分序列,并进行测序。[结果]通过RT-PCR获得了一段基因序列。该PCR产物经1.0%琼脂糖凝胶电泳呈现出一条长为1584bp左右的特异性条带,与预期设计的1581 bp的扩增片段相符;与电子克隆得到的相应序列比对结果表明,两者一致性高达99.22%。其编码蛋白含有与酶功能密切相关的保守氨基酸序列,与其他物种的BADH具有较高的一致性。[结论]成功克隆了向日葵BADH基因的部分序列。[Objective] The research was to provide the theory and practical basis for the utilization of BADH Gene from Helianthus annuus. [Method] Partial sequence of sunflower BADH eDNA was obtained by the reverse transcription polymerase chain reaction （RT-PCR） with sili- co cloning technique. [Results] The partial gene sequence was obtained with RT-PCR. The PCR production was eleetrophoresed in 1.0% aga- rose gel, and a specific band of about 1584 bp length was observed, which size was similar to designed size. Sequence comparison of sequencing result revealed 99.22% nueleotide sequence identity with in silico cloning result. Protein deduced by sequencing result contained conserved amino acids residue and sequences closely related to function of enzyme, and possessed high amino acid sequence identity with BADH form other organism. [Conclusion] The oartial sumflower BADH gene seauence was successfully cloned.

关 键 词：向日葵 甜菜碱醛脱氢酶 BADH基因 基因克隆 

分 类 号：S188[农业科学—农业基础科学]