抗褐飞虱和抗除草剂转基因粳稻新品系的选育及其中间试验  

Breeding and field trial of a transgenic japonica rice line with resistance to brown planthopper and herbicide

作　　者：王玲[1,2] 于恒秀[1] 黄世文[2] 赵志鹏[1,3] 龚志云[1] 汤述翥[1] 顾铭洪[1] 刘巧泉[1] 

机构地区：[1]扬州大学教育部植物功能基因组学重点实验室,江苏扬州225009 [2]中国水稻研究所,浙江杭州310006 [3]上海市农业科学院,上海200106

出　　处：《扬州大学学报（农业与生命科学版）》2008年第3期23-27,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：江苏省高新技术项目(BG2002301);教育部“新世纪优秀人才支持计划”项目(NCET-07-0736);江苏省教育厅资助项目(k05015)

摘　　要：利用根癌农杆菌介导法,将位于同一双元载体pCUGNA-BAR上的GNA基因和Bar基因导入粳稻品种广陵香粳,获得了转基因品系。经PCR扩增检测和Southern杂交分析证明GNA基因已整合到转化植株的基因组中。在转化后代中选育了一个表现优异的转基因水稻新品系3015-1-1进行中间试验,田间农艺性状调查发现该品系3015-1-1基本保持了未转化对照优良的农艺特性。抗虫和抗除草剂试验表明,3015-1-1对褐飞虱的蜜露排泄量和繁殖率具有显著的抑制作用,对除草剂Basta有明显抗性。Both GNA and Bar genes,located at the same binary vector pCUGNA-BAR,were introduced into a japonica rice variety Guanglingxiangjing by Agrobacterium-mediated transformation.Several transgenic rice plants were regenerated,and the GNA gene was integrated into the genome of transgenic plants confirmed by PCR and Southern blotting analyses.In the field trial,the selected homozygous transgenic rice line 3015-1-1 preserved advanced agronomic traits of its non-transformed wild type.The resistance to insect and herbicide were carefully analyzed,the results showed that the transgenic rice line 3015-1-1 showed significant inhibition to honeydew excretion and fecundity of brown planthopper,and high resistance to the herbicide Basta.

关 键 词：转基因水稻 GNA基因 BAR基因 褐飞虱 除草剂 

分 类 号：S511.035.3[农业科学—作物学] Q785[生物学—分子生物学]