刺五加多糖和GST对实验性肝癌影响的病理学研究  

Pathoiogic Study on effect of Acanthopanax Santicosus Polysaccharides and GST during rats hepatocarcinogenesis

作　　者：张文智[1] 张乃哲[2] 陈秀鉴[3] 

机构地区：[1]河北省儿童医院,石家庄050031 [2]河北省医学科学院 [3]河北医科大学

出　　处：《肿瘤防治研究》1997年第2期78-80,F002,共4页Cancer Research on Prevention and Treatment

摘　　要：120只实验性大鼠在化学致癌物（3’－me－DAB）诱发肝癌基础上，通过对肝脏病理形态、γ-谷氨酰转肽酶（γ－GT）阳性细胞和肝组织内γ－GT含量的检测，观察利五加多糖和谷胱甘肽硫转移酶（GST）对肝细胞增生癌变过程的影响。结果表明1．刺五加组肝脏形态学与3’－me－DAB组无明显差异但肝脏发生病变的时间向后推迟了一周；肝细胞γ－GT阳性强度明显低于3‘－me－DAB组；肝组织γ－GT含量动态分析发现低于3’－me－DAB组且缓慢增加，实验第9天才有升高趋势（3’－me－DAB组第4天就迅速升高）。提示剩五加多糖对肿瘤性增殖有明显延缓作用。2．GST组病理形态和3’－me－DAB组无异；肝细胞γ－GT阳性强度及府组织γ－GT含量明显增加，高于3’－me－DAB组，特别是实验后34天出现持续升高态直至实验结束。提示GST皮下免疫法未能起到保肝解毒作用。本文对利五加的研究初步表明有抑瘤作用，增加实验剂量进一步的研究是必要的。The effect of Acanthopanax Santicosus polysaccharides (ASPS) and Glutathione S-transferase(GST)for hepatic cellular morphology, the number of γ-Glutamyl transpeptiase (γ-GT) positive cells and γ-GT tissue content were studed in 148 rat livers based on 3'-me-DAB hepatocarcinogenesis. Results show: 1, In APSP group, hepatomorphology are similar to 3'-me-DAB group and the number of γ-GT positive cells and γ-GT content of hepatic tissue slowly increase, with a lower proliferatic level than in 3'-me-DAB group; In Apsp group, 9 day after experimenl show pathologic Variety, but in 3'-me-DAB group 4 day; These data demonstrate ASPS have distinct retardant effect to neoplastic proliferation 2, pathomorphology in GST group are also similar with 3'-me-DAB group, and the number of γ-GT positive cells and γ-GT content indictate higher proliferatic level than 3'-me-DAB group. It suggestes the effect of hepatic detoxication in GST is suspected by appling subcutaneous immunomethod. Influnce on experimental hepatocarcinoma in ASPS make further study, incrensing ASPS dose be suggested.

关 键 词：刺五加 多糖 GST γ 谷氨酰转肽酶 肝肿瘤 

分 类 号：R735.705[医药卫生—肿瘤] R73－36[医药卫生—临床医学]