实时荧光定量RT-PCR对胃癌组织中hPMS2 mRNA的检测及其临床意义  

Detection of hPMS2 mRNA in gastric cancer using real-time fluorescent quantitative RT-PCR and its clinical significance

作　　者：徐光香[1] 刘希双[1] 杨堃[2] 

机构地区：[1]青岛大学医学院附属医院内镜诊治科,山东省青岛市266003 [2]青岛大学医学院附属医院分子生物学实验室,山东省青岛市266003

出　　处：《世界华人消化杂志》2008年第31期3510-3514,共5页World Chinese Journal of Digestology

基　　金：青岛市科技计划资助项目;No.06-2-2-5-nsh-3~~

摘　　要：目的:探讨DNA错配修复基因hPMS2在胃癌组织中的表达水平及其临床意义.方法:应用实时荧光定量逆转录聚合酶链反应(RT-PCR)技术对41例胃癌患者的癌组织、37例癌旁萎缩性胃炎组织、25例慢性萎缩性胃炎组织及20例慢性浅表性胃炎组织中hPMS2 mRNA进行定量检测,以三磷酸甘油醛脱氢酶基因(hGAPDH)为内参照.结果:胃癌组织、癌旁萎缩性胃炎组织、慢性萎缩性胃炎组织及慢性浅表性胃炎组织中hPMS2 mRNA的含量分别是28.33±14.05,16.88±10.67,7.25±8.72和1.78±1.34,四组相比差异有显著性(F=31.82,P=0.00).胃癌组织中hPMS2mRNA含量明显高于其他三组;癌旁萎缩性胃炎组织中含量明显高于非胃癌患者的胃炎组织,差异均有显著性(P<0.01);而非胃癌患者的慢性萎缩性胃炎组织中hPMS2 mRNA的含量与慢性浅表性胃炎组织相比差别无显著性.hPMS2 mRNA含量与肿瘤的大小、浸润深度、有无淋巴结转移关系不明显,差异均无显著性.结论:hPMS2基因的异常转录可能与胃癌的发生有关,而与胃癌的发展关系不大.AIM： To investigate the expression of DNA mismatch repair gene hPMS2 and its clinical significance in gastric cancer. METHODS： Real-time fluorescent quantitative reverse transcriptase polymerase chain reaction （RT-PCR） was used to detect the level of hPMS2 mRNA respectively in 41 cases of gastric cancer and 37 cancer-adjacent mucosa samples of atrophic gastritis and 25 cases of chronic atrophic gastritis and 20 cases of chronic superficial gastritis. The human glyceraldehydes-3-phosphate dehydrogenase （hGAPDH） gene was used as a reference for normalization of the expression level. RESULTS： The expression levels of hPMS2 mRNA in gastric cancer, the corresponding cancer-adjacent mucosa, chronic atrophic gas- tritis and chronic superficial gastritis were 28.33 ± 14.05, 16.88 ± 10.67, 7.25 ± 8.72 and 1.78 ±1.34, respectively. Significant differences were detected among the four groups（F=31.82,P=0.00）. The expression level of hPMS2 mRNA was markedly higher in gastric cancer than in the other tissues （P 〈 0.01）, and significantly higher in the corresponding cancer-adjacent rnucosa than in nonneoplastic mucosa （P 〈 0.01）, but no significant difference was detected between chronic atrophic gastritis and chronic superficial gastritis. The expression of hPMS2 in gastric cancer had no obviously relationship with the size, the degree of infiltration of tumor nor the metastasis of lymph node. CONCLUSION： The abnormal transcription of hPMS2 gene may be related to gastric cancer, but not involved in the development of gastric cancer.

关 键 词：胃癌 错配修复基因 hPMS2 MRNA 实时荧光定量逆转录聚合酶链反应 

分 类 号：R735.2[医药卫生—肿瘤]