Pim-3异常表达在胃癌中的意义  

Significance of aberrant Pim-3 expression in gastric carcinoma

作　　者：胡志方[1] 黄缘[1] 钟琼[1,2] 简捷[1] 

机构地区：[1]南昌大学第二附属医院消化内科江西省分子医学重点实验室,江西省南昌市330006 [2]江西省肿瘤医院,江西省南昌市330006

出　　处：《世界华人消化杂志》2008年第31期3515-3518,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨Pim-3的异常表达在胃癌发展过程中的作用.方法:使用半定量RT-PCR法和免疫组化法检测40例胃癌组织及20例癌旁正常组织标本Pim-3的表达,并比较Pim-3的表达与胃癌临床病理参数的关系.结果:与正常胃黏膜相比,Pim-3mRNA的表达量在胃癌组织中更高(0.287±0.058 vs 0.053±0.055,P<0.001).中分化腺癌中Pim-3蛋白的表达高于低分化腺癌组织中的表达,两者比较差异显著(50%vs20%,P<0.05).Pim-3的表达与淋巴转移、静脉转移密切相关(r=0.385,0.412,P=0.014,0.008).结论:Pim-3可作为胃癌早期的生物标志物,并可预示肿瘤的预后.AIM： To investigate the role of Pim-3 aberrant expression in the development of gastric carcinoma. METHODS： Semi-quantitative RT-PCR method and immunohistochemistry method were used to detect the expression of Pim-3 in 40 gastric carcinoma tissues and 20 adjacent normal tissues. We also compared relationship between Pim-3 expression and the clinicopathological parameters of gastric carcinoma. RESULTS： Pim-3 mRNA expression was enhanced in gastric carcinoma compared with noncancerous mucosa （0.287 ± 0.058 vs 0.053 ± 0.055, P 〈 0.001）. Protein expression of Pim-3 was significantly higher in moderately differentiated adenocarcinoma tissue than in poorly differentiated adenocarcinoma tissue. Pim-3 expression was closely correlated with lymphatic metastasis and venous invasion （r = 0.385, 0.412, P = 0.014, 0.008）. CONCLUSION： Pim-3 can be seen as the biological marker for early phase of gastric carcinoma and a prognostic factor to predict the prognosis of cancer.

关 键 词：PIM-3 胃癌 异常表达 逆转录酶链式反应 免疫组化法 

分 类 号：R735.2[医药卫生—肿瘤] R734.2[医药卫生—临床医学]