内质网分子伴侣糖调节蛋白94在非酒精性脂肪肝中的表达及意义  

Expression of endoplasmic reticulum molecular chaperone GRP94 in human nonalcoholic fatty liver and its clinical significance

作　　者：俞泽元[1] 高鹏[2] 申力[2] 黄海云[2] 但杰[1] 

机构地区：[1]兰州大学临床医学院,甘肃省兰州市730000 [2]甘肃省人民医院普外科,甘肃省兰州市730000

出　　处：《世界华人消化杂志》2008年第31期3566-3570,共5页World Chinese Journal of Digestology

摘　　要：目的:检测非酒精性脂肪肝患者肝组织中糖调节蛋白94的表达情况,探讨非酒精性脂肪肝病(nonalcoholic fattyliver disease,NAFLD)的发病机制.方法:应用半定量逆转录聚合酶链反应(RT-PCR)法和免疫组化法检测脂肪肝组和正常对照组肝组织中糖调节蛋白94基因和蛋白表达,HE染色观察肝脏脂肪变性、炎性活动和坏死,并进行相关分析.结果:非酒精性脂肪肝组脂肪变性、炎症评分及坏死评分较正常对照组明显增加,差异具有统计学意义(3.89±0.32 vs 0.20±0.4;3.67±0.49 vs 0;0.44±0.51 vs 0,P<0.01或0.05).与正常组比较,脂肪肝患者肝组织糖调节蛋白94mRNA和蛋白表达明显增强(0.86±0.02 vs 0.37±0.03;0.34±0.01 vs 0.31±0.01,均P<0.01).糖调节蛋白94mRNA和蛋白表达与脂肪变性、炎症评分及坏死评分明显呈正相关(P<0.01).结论:非酒精脂肪肝引起的肝脏损害可能与糖调节蛋白94表达有关.AIM： To detect the expression of GRP94 in human nonalcoholic fatty liver injury, and to explore the mechanism of human nonalcoholic fatty liver disease （NAFLD）. METHODS： Using immunohistochemistry and RT-PCR, GRP94 protein and mRNA expression were detected in fatty liver specimens and nor- mal liver specimens. Hepatic steatosis, inflammation and necrosis were graded by routine H＆E staining of liver sections, and their correla- tions were analyzed. RESULTS： Steatosis, inflammation and necrosis were markedly increased in nonalcoholic fatty liver group than in the control group （3.89 ± 0.32 vs 0.20 ± 0.4; 3.67 ± 0.49 vs 0; 0.44 ± 0.51 vs O, P 〈 0.01 or 0.05）. The expression of GRP94 mRNA and its protein expression were enhanced significantly in nonalcoholic fatty liver group than in the normal control group （0.86 ± 0.02 vs 0.37 ± 0.03; 0.34 ± 0.01 vs 0.31 ± 0.01, both P 〈 0.01）. The expression of GRP94 mRNA and its protein expression were positively associated with steatosis, inflammation and necrosis scores （P 〈 0.01）. CONCLUSION： The excessive expression of GRP94 could participate in the development of nonalcoholic fatty liver disease.

关 键 词：非酒精性脂肪肝 糖调节蛋白94 免疫组织化学 半定量逆转录聚合酶链反应 

分 类 号：R575.5[医药卫生—消化系统] R735.1[医药卫生—内科学]