精子蛋白17异常表达增强卵巢癌细胞HO-8910迁移性  

Aberrant Expression of Sperm Protein 17 Enhances Migration of Ovarian Cancer Cell Line HO-8910

作　　者：刘群[1] 李芳秋[1] 韩艳玲[1] 王立魁[1] 侯亚义[2] 

机构地区：[1]南京大学医学院临床学院/南京军区南京总医院解放军临床检验医学研究所,江苏南京210002 [2]南京大学医学院,江苏南京210093

出　　处：《中华男科学杂志》2008年第11期982-986,共5页National Journal of Andrology

基　　金：国家自然科学基金(30670599);国家973计划(2006CB705707);南京军区医学科学技术“十一五”研究计划重点项目(06Z43)

摘　　要：目的:探讨精子蛋白17(sperm protein 17,Sp17)异常表达对人卵巢癌细胞HO-8910迁移性的影响。方法:应用脂质体转染法将携带Sp17和增强型绿色荧光蛋白基因的重组质粒pEGFP-Sp17转染卵巢癌细胞HO-8910,RT-PCR及Western印迹法检测Sp17的表达情况;用Transwell小室法检测细胞迁移能力的改变。结果:重组质粒pEGFP-Sp17转染HO-8910细胞后,Sp17与EGFP以融合蛋白形式表达;重组质粒转染组与空载体转染组发生迁移的细胞数分别为(156.6±14.9)个/高倍视野和(39.3±8.53)个/高倍视野,差异具有显著性意义(P<0.05)。结论:Sp17基因异常表达明显增强卵巢癌细胞HO-8910迁移能力。Objective ： To explore the effects of aberrant expression of sperm protein 17 （Spl7） on the migration of the ovarian cancer cell line HO-8910. Methods： The recombinant plasmid pEGFP-Spl7 containing Sp17 and enhanced green fluorescent protein gene was transfected into the human ovarian cancer cell line HO-8910 with Lipofeetamine 2000. The expression of Sp17 was examined by RT-PCR and Western blot, and the cell migratory capability detected by Transwell chamber assays. Results ： Sp17 was expressed as a fusion protein with EGFP after transfected. There was a significant difference in the migratory cell number of the transfected and the control cells （ 156.6 ＋ 14.9/HP vs 39.3 ＋ 8.53/HP, P 〈 0.05 ）. Conclusion ： The aberrant expression of Sp17 greatly enhances the migration of ovarian cancer cells.

关 键 词：精子蛋白17 卵巢癌 迁移 

分 类 号：R737.31[医药卫生—肿瘤]