环麦芽糊精葡聚糖转移酶高活性的土壤微生物菌种筛选  

Screening of High Cyclodextrin Glucanotransferase Productive Microbial Strains

作　　者：袁普伟[1,2] 黄建新[1] 王璋[2] 

机构地区：[1]西北大学生命科学学院,陕西西安710069 [2]中国食品发酵工业研究院,北京100027

出　　处：《食品与发酵工业》2008年第10期67-69,共3页Food and Fermentation Industries

摘　　要：根据环麦芽糊精葡聚糖转移酶(CGTase)催化淀粉和糊精生成环状糊精(CD),环状糊精可以吸附筛选培养基中的刚果红和甲基橙使菌落周围形成透明圈,且透明圈越大,透明度越好,CGTase的转糖基活性越高的酶催化特性,设计筛选模版,进行产酶菌株分离筛选。经初筛、复筛得到了YS-19和YS-40的2株转糖基活性较高CGTase菌种,酶活分别为2.90U/mL和3.15U/mL。Cyclodextrin glucanotransferase （CGTase） is a unique enzyme that catalyzes the conversion of starch and dextrin to cyclodextrin （CD）, which absorbs congo red and methyl orange contained in the selective medium and forms clear and transparent color zone. The size and the clearance of the transparent zone can refer the activity of CGTase transglucosylation. After the primary isolation and secondary screening, 2 strains named YS-19 and YS-40 with high transglucosylation potential were obtained. Their activity were 2.90U/mL and 3.15U/mL, respectively.

关 键 词：环麦芽糊精葡聚糖转移酶 分离筛选 土壤微生物 透明圈 

分 类 号：Q93[生物学—微生物学]