反相高效液相色谱法测定富硒脱水菜心中的硒代氨基酸  

Determination of Seleno-amino Acids in Enriched-selenium Dehydrated Brassica Chinensis by RHPLC

作　　者：潘红阳[1] 王树英[1] 莫海珍[2] 

机构地区：[1]江南大学食品科学与技术国家重点实验室,硕士工程师江苏无锡214122 [2]江南大学食品学院,江苏无锡214122

出　　处：《食品与发酵工业》2008年第10期141-144,148,共5页Food and Fermentation Industries

摘　　要：建立了柱前衍生反相高效液相色谱-紫外检测富硒脱水菜心中硒代胱氨酸(SeCys)、硒甲基半胱氨酸(SeMeCys)和硒代蛋氨酸(SeMet)的方法,优化了样品预处理方法,并评价其定量分析的线性、准确性、重复性和检出限,结果显示:以0.1mol/LHCl为提取溶剂,超声处理8h,提取效果最佳;3种硒代氨基酸的平均加标回收率为95.7%～112.9%;在各硒代氨基酸的线性范围内,线性关系良好(R>0.999);保留时间和峰面积重复性的相对标准偏差(RSD)均分别低于2.0%和5.0%;酸性条件下的检出限为0.004～0.009mg/L。A reversed phase high performance liquid chromatography/variable wavelength detector with precolumn derivatization was developed for the simultaneous determination of selenocystine（SeCys）, seleno-methylcysteine（SeMeCys） and selenomethionine（SeMet）. This method was validated for linearity, accuracy, repeatability and limits of detection （LOD）. The results showed that average recoveries of seleno-amino acids standards ranged from 95.7% to 112.9%, linearity was good （R^2〉0.999）; the relative standard deviations of repeatability for retention time and peak area were less than 2. 0% and 5.0%, respectively; LOD was 0. 004～0. 009 mg/L in acid condition. The method is simple, accurate, and sensitive and reliable for quality control of enriched--selenium dehydrated Brassica Chinensis.

关 键 词：富硒脱水菜心 反相高效液相色谱 硒代氨基酸 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]