Osteoglycin高表达降低小鼠肝癌Hca-F细胞淋巴环境中MMPs分泌能力  

High Expression of Osteoglycin Decreases MMP Activity of Murine Hepatocarcinoma Hca-F Cells Cultured with Extract of Lymph Node

作　　者：崔晓楠[1] 唐建武[2] 候力[2] 宋波[2] 王波[2] 

机构地区：[1]大连医科大学附属一院肿瘤科,辽宁省大连市116011 [2]大连医科大学病理教研室,辽宁省大连市116011

出　　处：《中国肿瘤临床》2008年第22期1303-1306,1313,共5页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金资助(编号:30500586)~~

摘　　要：目的:研究Osteoglycin表达与肿瘤细胞分泌基质金属蛋白酶(matrix metalloproteinases,MMPs)的关系。方法:构建真核表达载体pIRESpuro3 Osteoglycin(+),将其转染至高淋巴道转移能力小鼠肝癌Hca-F细胞,接种于615小鼠皮下,体内试验评价转移能力;同期采用酶谱法体外试验评价不同环境中Hca-F细胞表达Osteoglycin与其分泌MMPs的关系。结果:转染pIRESpuro3 Osteoglycin(+)的Hca-F细胞Osteoglycin在mRNA和蛋白水平表达显著增高,体内试验显示,其外周淋巴结转移率明显降低(P=0.028);体外试验表明,转染Osteoglycin基因显著降低了Hca-F细胞淋巴环境中MMPs分泌能力(P<0.05或P<0.01),然而未能影响肝匀浆或脾匀浆环境或DMEM培养基中肿瘤细胞MMPs分泌能力。结论:高表达Osteoglycin降低小鼠肝癌Hca-F细胞淋巴环境中MMPs分泌能力,抑制肿瘤细胞MMPs分泌能力可能是Osteoglycin调控肿瘤淋巴道转移机制之一。Objective： To investigate the possible correlation between osteoglycin expression and matrix metalloproteinase （MMP） activity in tumor cells. Methods： A eukaryotic expression plasmid plRESpuro3 osteoglycin （＋） was constructed and transfected into mouse hepatocarcinoma Hca-F cells with high metastatic potential. In vivo tumor metastasis assay was employed to evaluate the contribution of osteoglycin expression to the malignant behavior of Hca-F and zymographic analysis was used to observe the possible correlation between osteoglycin expression and MMP activity of Hca-F cells cultured with different condition. Results： Hca-F cells transfected with plRESpuro3 osteoglycin （＋） produced effective and stable expression of osteoglycin at mRNA and protein levels. Lymphatic metastasis rate was decreased in tumor burden mice （P=-0.028）. High expression of osteoglycin decreased MMPs activity of Hca-F cells cultured with extract of lymph node （P〈0.05 or P〈0.01） but didn＇t influence MMPs activity of Hca-F cells cultured with liver or spleen extract or DMEM medium. Conclusion： High expression of osteoglycin can decrease MMPs activity in Hca-F cells cultured with extract of lymph node. Regulation of MMPs activity might be one of the mechanisms that osteoglycin can suppress lymphatic metastasis.

关 键 词：OSTEOGLYCIN 转染 肝肿瘤 肿瘤转移 MMPS 

分 类 号：R735.7[医药卫生—肿瘤]