猪Lmbr1基因部分cDNA序列的克隆及序列分析  

Cloning and Analysis of Partial cDNA Sequence of Lmbr1 Gene in Pigs

作　　者：王金勇[1] 白小青[1] 赵献之[1] 范守城[2] 李琴[1] 刘文[1] 

机构地区：[1]重庆市畜牧科学院,重庆402460 [2]重庆市科学技术信息中心,重庆401147

出　　处：《畜牧兽医学报》2008年第11期1616-1620,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：重庆市重点自然基金(CSTC2005BA1009);重庆市科技攻关重大项目(CSTC2006AA1008)

摘　　要：利用RT—PCR和RACE方法克隆了正常猪和全同胞多趾猪Lmbr1基因部分cDNA序列并进行了序列分析。结果表明，正常猪该序列长1797bp，其中CDS序列1178bp，3’UTR序列619bp。全同胞多趾猪该序列长1069bp，其中CDS序列768bp，3’UTR序列301bp。正常猪与多趾猪Lmbr1基因的CDS序列相似性近77％，而3’UTR序列相似性不足20％。两者的CDS序列在755～768bp间，共发生13个核苷酸突变：G755C、A756T、A757G、T758C、C759A、A760G、C761T、T762G、A763C、G764T、A765G、T767G、T768A。其中，前11个突变属于错义突变，导致相应的氨基酸序列中4个氨基酸发生变化：G突变为A、I突变为A、T突变为V、R突变为L；后2个突变属于无义突变，导致阅读框提前终止。猪Lmbrl基因发生突变能引起SHH的异常表达，这可能是导致猪多趾发生的根本原因。In this study we cloned partial cDNA sequences of Lrnbrl gene from a normal pig and a polydactyly pig by RT-PCR and RACE and analyzed the sequences. The results showed that the cDNA sequence from the normal pig was 1 797 bp,including 1 178 bp in CDS and 619 bp in 3＇ UTR. The cDNA sequence from the polydactylous pig was 1 069 bp,including 768 bp in CDS and 301 bp in 3＇UTR. the CDS sequences similarity of Lrnbrl gene was near 77% between the normal pig and the polydactyly pig, while the 3＇UTR sequences similarity was below 20%. There were 13 mutations among 755-768 base pairs between the CDS sequences, including G755C, A756T, A757G, T758C, C759A, A760G, C761T, T762G, A763C, G764T, A765G, T767G and T768A. The former 11 mutations belonged to missense mutations, which resulted in 4 amino acids change, including G→ A, I→ A, T → V and R →L. The later 2 mutations belonged to nonsense mutations,which resulted in the termination of ORE in advance. It is presumed that the fundamental reason for the form of the polydactyly pig is the mutations of Lmbr1 gene, which could result in the exceptional expression of SHH.

关 键 词：猪 Lmbr1基因 RACE 突变 

分 类 号：S828[农业科学—畜牧学] Q591.3[农业科学—畜牧兽医]