原癌基因c-fos、p53及运动神经诱向因子1受体在人早期胎盘绒毛的免疫组织化学定位  

IMMUNOHISTOCHEMICAL LOCALIZATION OF c fos, p53 PROTEIN AND MOTONEURONOTROPHIC FACTOR 1 RECEPTOR IN EARLY HUMAN PLACENTAL VILLI

作　　者：黄威权[1] 孙岚[1] 

机构地区：[1]第四军医大学组织学胚胎学教研室

出　　处：《解剖学报》1997年第4期404-406,I013,共4页Acta Anatomica Sinica

摘　　要：为了解原癌基因对胎盘滋养层细胞的影响，用原癌基因ｃ－ｆｏｓ、ｐ５３蛋白的抗体和运动神经诱向因子１（ｍｏｔｏｎｅｕｒｏｎｏｔｒｏｐｈｉｃｆａｃｔｏｒ１，ＭＮＴＦ１）抗独特型抗体的免疫组织化学技术，研究了ｃ－ｆｏｓ、ｐ５３及ＭＮＴＦ１受体在人早期胎盘的分布。人早期胎盘的两类滋养层细胞及绒毛中轴的基质细胞均呈ｃ－ｆｏｓ、ｐ５３免疫反应性，反应产物大部分布于胞核内。在相邻切片上，ｃ－ｆｏｓ免疫反应性细胞同样显示ＭＮＴＦ１受体免疫反应性，其反应物质分布于胞质内。以上结果提示，原癌基因ｃ－ｆｏｓ和ｐ５３可能参与调控绒毛细胞的增殖分化，而且ｃ－ｆｏｓ和ＭＮＴＦ１受体可能有协同调控作用。n order to study how the oncogenes affect the placental trophoblast cells, immunohistochemical localization of c fos, p53 protein and motoneuronotrophic factor 1 (MNTF1) receptor in human early placenta were analyzed by using immunohistochemical technique. c fos and p53 proteins were found to be localized in syncytiotrophoblasts, cytotrophoblasts and villous stroma cells. The immunohistochemical double labeling on adjacent section showed that syncytiotrophoblasts, cytotrophoblasts and villous stroma cells were also MNTF1 receptor positive. These results suggested that c fos oncogene and p53 may play a crucial role in inducing proliferation and differentiation of villous cells in human placenta, and MNTF1 receptor may play a cooperative role in the inducing process.

关 键 词：免疫组织化学 MNTF1 胎盘 P53基因 C-FOS基因 

分 类 号：R730.231.3[医药卫生—肿瘤]