狗精索内淋巴管注射癌细胞悬液后对淋巴结切片的观察和图像分析  

OBSERVATION AND IMAGE ANALYSIS ON LYMPH NODESECTIONS AFTER INJECTING CANCER CELL SUSPENSIONINTO CANINE INTERNAL SPERMATIC LYMPHATICS

作　　者：谭玉珍[1] 王海杰[1] 王新明[1] 

机构地区：[1]潍坊医学院组织学胚胎学教研室

出　　处：《解剖学报》1997年第4期433-437,I019,共6页Acta Anatomica Sinica

摘　　要：通过淋巴管内注射肿瘤细胞以探讨肿瘤细胞转移至左锁骨上淋巴结的途径。在狗睾丸内注射叶绿素溶液的同时，向精索内淋巴管注射小鼠骨髓瘤细胞或人胃腺癌细胞悬液，２ｈ后取显色的纵隔淋巴结和颈部淋巴结，做石蜡切片。显微镜下观察癌细胞在淋巴结内的分布，并应用定量电视显微镜（Ｑｕａｎｔｉｍｅｔ９７０）图像分析系统测量癌细胞核的形态学参数和ＤＮＡ含量。结果发现叶绿素和癌细胞分布于淋巴结的被膜下窦和小梁周窦，但髓窦和输出淋巴管内无叶绿素和癌细胞，结果表明：叶绿素和癌细胞是经输入淋巴管进入淋巴结的，癌细胞可经胸导管及其侧支顺淋巴流进周围淋巴结，并引起淋巴结肿大。ancerometastasis mechanism of supraclavicular lymph nodes is not well known. The passway along which cancer cells metastasize to supraclavicular lymph nodes was investigated with intralymphatic injection of cancer cells. The locationi of cancer cells in the lymph nodes was observed under light microscope. The nucleus parameter and DNA content of the cancer cells were measured by Quantimet 970 image analyser. It was found that cancer cells were located in subcapsular sinuses and peritrabecular sinuses of the lymph nodes, while no cells were seen in medullary sinuses and efferent lymphatic vessels. Our experiments demonstrated that cancer cells enter lymph nodes through afferent lymphatic vessels. These results suggest that cancer cells may enter lymph nodes following lymph flow from the thoracic duct and its collateral branches and cause the tumefaction of these lymph nodes.

关 键 词：叶绿素 淋巴管 淋巴结 肿瘤细胞 转移 

分 类 号：R73-37[医药卫生—肿瘤]