内氏放线菌细胞壁成分诱导人成纤维细胞产生白细胞介素-1β、-6和肿瘤坏死因子α的研究  

Production of interleukin-1β, interleukin-6 and tumor necrosis factor α in cultured human fibroblast with stimulation of abstract from Actinomyces naeslundii ATCC19246

作　　者：赵丽娟[1] 李文[2] 郑燕[3] 

机构地区：[1]大连大学附属新华医院口腔科,辽宁大连116021 [2]四川大学华西口腔医院牙体牙髓科,四川成都610041 [3]大连大学医学院口腔教研室,辽宁大连116600

出　　处：《华西口腔医学杂志》2008年第5期473-474,478,共3页West China Journal of Stomatology

基　　金：大连市卫生局科研基金资助项目(2004);辽宁省教育厅科研基金资助项目(20040044)

摘　　要：目的探讨内氏放线菌菌株ATCC19246细胞壁成分能否诱导人成纤维细胞产生白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子α(TNFα)。方法采用提取脂磷壁酸(LTA)的方法提取内氏放线菌菌株ATCC19246细胞壁成分,用3种质量浓度(分别为1、10、100mg/mL)提取物分别刺激人成纤维细胞THP-1细胞株,用ELISA方法测定细胞培养上清中的IL-1β、IL-6、TNFα。结果THP-1细胞株可以产生一定量的IL-1β、IL-6、TNFα,以质量浓度为10mg/mL时诱导产生的量最高。结论内氏放线菌细胞壁提取物可以诱导THP-1细胞株产生IL-1β、IL-6、TNFα,但随着质量浓度变化产生的量存在差异。Objective To observe the production of interleukin（IL）-1β, IL-6 and tumor necrosis factor α（TNFα） from stimulated human fibroblast with abstract from cell wall of Actinomyces naeslundii ATCC19246. Methods The abstract from the cell wall from Actinomyces naeslundii were extracted and purified with the method of purifying lipoteichoic acid（LTA） and stimulated the THP-1 with three different concentrations （1, 10, 100 mg/mL）. The level of IL-1β, IL-6 and TNFα in the supernatant was quantitatively analyzed by ELISA. Results Abstracts at the concentrations of 10, 100 mg/mL significantly produced IL-1β, IL-6 and TNFα, especially 10 mg/mL. Conclusion The abstract from cell wall of Actinomyces naeslundii may significantly increase IL-1β, IL-6 and TNFα level in the supernatant of THP-1, and the increasing level is different with the concentrations.

关 键 词：内氏放线菌 白细胞介素 肿瘤坏死因子Α 成纤维细胞 

分 类 号：R780.2[医药卫生—口腔医学]