尼莫地平对人牙本质形成的影响  

Effects of nimodipine on human dentinogenesis

作　　者：朱晓茹 张蓉 李玉成 唐荣银 赵守亮[2] 

机构地区：[1]第四军医大学口腔医院牙体牙髓病科,陕西西安710032 [2]同济大学口腔医院口腔内科,上海200072

出　　处：《华西口腔医学杂志》2008年第5期563-566,共4页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30471889)

摘　　要：目的采用人牙齿切片体外器官培养的方法研究尼莫地平对牙本质形成的影响。方法收集年轻人健康前磨牙,用慢速切锯制备2mm厚的牙齿横切片,将在尼莫地平溶液中浸泡过的琼脂糖微球与在PBS液中浸泡过的琼脂糖微球对称放置在牙片上,将切片用含琼脂糖的半固体培养基包被后采用器官培养方法培养1周,观察其对牙本质形成的影响。结果尼莫地平处理后四环素线状荧光带较对照组变细、变淡,Von-Kossa染色前期牙本质处球状矿化也较对照组减少,透射电镜显示实验组成牙本质细胞内的分泌小泡与对照组相比略有增多,但是Ⅰ型胶原免疫组化染色显示实验组和对照组无明显区别。结论尼莫地平能够影响牙本质的矿化形成,但对牙本质基质的合成和分泌无明显影响。Objective Studies have showed that L type calcium channel plays an important role in dentin calcification and affects tooth development and tooth reparation after injury. The objective of this article is to study the effects of nimodipine, blocking agent of L type calcium channel, on human dentinogenesis using human tooth slice organ culture in vitro. Methods Young healthy human premolars were collected, and cut into 2 nun-thick transverse slices by low speed diamond saw. Agarose beads dipped in nimodipine solution and PBS weresy mmetrically placed on tooth slices, and the slices were then embedded in a semisolid agarose-based medium and cultured with organ culture method for 1 week. Fluorescent band of tetracycline, Von-Kossa staining, immunohistochemical staining of the slices and transmission electron microscopy （TEM） of odontoblasts were observed to evaluate dentinogenesis changes of the slices. Results Tooth slices were successfully cultured in vitro for 1 week and the odontoblasts could maintain their original morphology. After treatment with nimodipine, the fluorescent band of tetracycline was narrow and weak, and globular calcification in predentine was decreased compared with the control. TEM showed that secretory vesicles in odontoblast were somewhat increased, but immunohistochemical staining for collagen I showed no difference between the two groups. Conclusion Nimodipine can influence the calcification of dentine, but has no obvious influence on the synthesis and secretion of dentine matrix. The results show that L type calcium channel is important in dentin calcification.

关 键 词：器官培养 尼莫地平 矿化 牙本质形成 

分 类 号：R78[医药卫生—口腔医学]