Development of SSR markers from ESTs of gramineous species and their chromosome location on wheat  

作　　者：Linzhi Li Junjun Wang Ying Guo Fangshan Jiang Yunfeng Xu Yingying Wang Haitao Pan Guanzhu Han Ruijun Li Sishen Li 

机构地区：[1]National Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai＇an 271018, China

出　　处：《Progress in Natural Science:Materials International》2008年第12期1485-1490,共6页自然科学进展·国际材料（英文版）

基　　金：supported by the Major State Basic Research Development Program of China (Grant No.2006CB101700);the National Natural Science Foundation of China (Grant No.3057155)

摘　　要：A total of 407,663 expressed sequence tags （ESTs） of wheat, barley, maize, rice, and sorghum, obtained from GenBank/dbEST, were used to search for simple sequence repeats （SSRs）. A total of 10,253 EST-SSRs, which accounted for 2.52% of all the ESTs, were identified. Using Primer Premier 5.0, 1367 EST-SSR primer pairs were designed, of which 715 with high quality were synthesized. The 715 primer pairs were tested on wheat, rice, maize, cotton, and soybean under the same PCR conditions, and the effective primer pairs in the five crops were 500 （69.93%）, 383 （53.57%）, 452 （63.22%）, 357 （49.93%）, and 388 （56.27%）, respectively. This indicated a high transferability of EST-SSR markers between far-ranging species. In addition, 139 EST-SSR primer pairs with 240 loci were localized on all the 21 wheat chromosomes by using Chinese Spring nulli-tetrasomic lines of wheat.407,663 的一个总数表示了小麦，大麦，玉米，米饭，和蜀黍的顺序标签(EST ) ，从 GenBank/dbEST 获得了，被用来寻找简单顺序重复(SSR ) 。10,253 EST-SSRs 的一个总数，说明了 2.52% 所有 EST，被识别。用教材首相 5.0， 1367 EST-SSR 教材对被设计，哪个 715 与高质量被综合。 715 教材对在五庄稼在一样的 PCR 条件，和有效教材对下面在小麦，米饭，玉米，棉花，和大豆上被测试是 500 (69.93%)， 383 (53.57%)， 452 (63.22%)， 357 (49.93%)，并且 388 (56.27%)分别地。这显示了在扩及远距离的种类之间的 EST-SSR 标记的高可转移性。另外，有 240 loci 的 139 EST-SSR 教材对在由使用小麦的中国春天 nulli-tetrasomic 线的所有 21 个小麦染色体上是局部性的。

关 键 词：EST-SSR WHEAT Maize Rice BARLEY SORGHUM Cotton Soybean 

分 类 号：S5[农业科学—作物学]