压力对体外培养牛眼小梁细胞的影响  

The effects of pressure on cultured bovine trabecular meshwork cells

作　　者：姜发纲[1] 魏厚仁[1] 吕源淑[1] 周元清 张缨[1] 

机构地区：[1]同济医科大学附属协和医院眼科

出　　处：《中华眼科杂志》1997年第6期410-412,I025,共4页Chinese Journal of Ophthalmology

摘　　要：目的观察压力对小梁细胞的影响。方法对体外培养牛眼小梁细胞施以不同程度的压力，用倒置相差显微镜、光镜、电镜观察细胞形态结构及吞噬功能的变化。结果细胞承受２．００ｋＰａ（１ｋＰａ＝７．５ｍｍＨｇ）、２．６７ｋＰａ压力４８小时，各项指标与对照组比较，差异无显著性。４．００ｋＰａ２４小时，细胞形态结构有轻度的损伤，吞噬功能有轻度下降。当压力进一步增加，时间更加延长后，细胞的损伤也更重。结论小梁细胞只能承受一定范围内的压力；高于这一范围的压力，将破坏细胞结构并影响其功能。Objective To observe the effects of pressure on trabecular meshwork cells. Methods Bovine trabecular meshwork cells were cultured and submitted to different amounts of hydrostatic pressure. Cellular morphology and phagocytic function were observed under inverted phase contrast microscope , light microscope and electron microscope. Results Compared with the control group, the cells under 2.0 kPa or 2.67 kPa for 48 hours had no remarkable difference in criteria observed. Those under 4.0 kPa for 24 hours showed slight changes in structure and a mild decrease in phagocytic function. The damage appeared more severe if the pressure was higher or lasted longer. Conclusion Trabecular meshwork cells can only bear pressure below a certain level. They may be destroyed structurally or impaired functionally by pressure over this level.

关 键 词：小梁细胞 压力 青光眼 细胞培养 体外培养 

分 类 号：R775.02[医药卫生—眼科]