南海真菌代谢物对前列腺癌细胞DU-145的影响  

Effect of marine fungal metabolites from the South China Sea on prostate cancer cell line DU-145

作　　者：林武[1,2] 方乐堃[1,2] 刘嘉伟[1,2] 程蔚琪[1,2] 云苗[1,2] 杨惠玲[1] 

机构地区：[1]中山大学中山医学院病理生理学教研室,广东广州510080 [2]中山大学中山医学院,广东广州510080

出　　处：《国际内科学杂志》2008年第10期562-563,F0003,共3页International Journal of Internal Medicine

基　　金：广东省科技计划项目(2007B030300002)

摘　　要：目的观察南海真菌代谢物1386A对雄激素非依赖性前列腺癌DU-145细胞增殖的影响。方法体外培养前列腺癌DU-145细胞,MTT法测定不同浓度1386A作用后细胞的生长情况。结果DU-145细胞在不同浓度1386A(40、20、10、5μmol/L)作用24、48、72h后,生长抑制率分别为61%、43%、30%、5%和91%、84%、63%、23%以及99%、96%、81%、52%。1386A作用DU-145细胞24、48、72h后的半抑制率(IC50)分别是25.31、8.62、4.79μmol/L。结论南海真菌代谢物1386A对雄激素非依赖性前列腺癌DU-145细胞具有抑制作用,且存在一定的剂量和时间依赖关系。Objective To observe the effect of marine fungal metabolites 1386A from the South China Sea on proliferation of androgen independent prostatic cancer cell line DU-145. Methods To culture DU-145 Cells in vitro and detect their growth conditions by MTT assay after being incubated with 1386A. Results After being incubated with different concentration of 1386A（40, 20, 10, 5 μmol/L）, the growth inhibiting rates of DU- 145 cells were 61%, 43%, 30%, 5% respectively（24 h） , 91%, 84%, 63%, 23% （48 h） and 99%, 96%, 81%, 52% respectively（72 h）. Half maximal inhabitory concentration（ IC50） of 1386A incubating DU- 145 ceils for 24, 48 and 72 h were 25.31, 8.62, 4. 79 μmol/L respectively. Conclusion Marine fungal metaholites 1386A from the South China Sea has inhibitive effect, which is dose-and time- dependent, on androgen independent prostatic cancer cell line DU-145.

关 键 词：南海真菌代谢物 雄激素非依赖性前列腺癌 细胞培养 

分 类 号：R737.25[医药卫生—肿瘤]