免疫化疗对晚期食管癌患者免疫细胞的影响  

The effect of immunochemotherapy on the immunocyte in advanced esophageal cancer

作　　者：王刚[1] 季楚舒[1] 段巧虹[1] 范平生[2] 何义富[1] 胡冰[1] 

机构地区：[1]安徽医科大学附属省立医院,安徽省立医院肿瘤内科,合肥230001 [2]安徽省肿瘤医院肿瘤内科

出　　处：《中国临床保健杂志》2008年第6期595-597,共3页Chinese Journal of Clinical Healthcare

基　　金：安徽省科技厅资助课题(06023090C);安徽省自然科学基金(007413256X)

摘　　要：目的探讨在晚期食管癌患者当中,免疫化疗对外周血免疫细胞所引起的变化情况。方法晚期食管癌患者48例,分为单独化疗组、化疗+长期免疫治疗组、化疗+短期大剂量免疫治疗组三组,治疗前后测定CD3+CD8+T细胞亚群、CD3+CD4+T细胞亚群、CD4+CD25+Treg细胞和NK细胞,化疗前后相关免疫细胞变化采用配对t检验;单因素方差分析检验不同治疗组间相关免疫细胞变化情况的区别。结果单独化疗组、化疗+长期免疫治疗组、化疗+短期大剂量免疫治疗组三组间CD3+CD8+T细胞亚群,CD3+CD4+T细胞亚群以及NK细胞比例治疗前后没有明显变化(P>0.05),而治疗前后患者CD4+CD25++Treg(高表达CD25的CD4+CD25+调节性T细胞)比例均下降(P<0.05);CD4+CD25+Treg细胞比例在单独化疗组(P=0.035)和化疗联合长期免疫治疗组(P=0.039)中,治疗后较治疗前有所上升;三组间治疗前后CD4+CD25+Treg上升比例、CD4+CD25++Treg下降比例变化没有统计学差别,其相关P值分别为P=0.86,P=0.14。结论免疫化疗在晚期食管癌治疗的过程中在改善免疫状态方面不优于单独化疗。Objective To investigate the change of immunocyte cell in patients with peripheral blood after chemotheropy in patients with advanced esophageal cancer.Methods Forty-eight advanced esophageal cancer patients were investigated from June 2006 to December 2007 in Anhui Provincial Hospital and Anhui Tumor Hospital,who were divided into chemotherapy group,chemotherapy ＋ permanent immunity therapy group and chemotherapy ＋ short term immunity therapy of large dose group.CD3^＋CD8^＋T cell、CD3^＋CD4^＋T cell、CD4^＋CD25^＋Treg cell 、and NK cell,in peripheral blood of esophageal cancer patients fore-aft therapy,were determined by flow cytometer.The change of immunocyte fore-aft therapy was analyzed by paired t-test.The change of immunocyte in different group was analyzed by one-factor analysis of variance.Results There was no difference between the proportion of CD3^＋CD8^＋T cell、CD3^＋CD4^＋T cell and NK cell before and after chemotherapy（P〉0.05） in chemotherapy group,chemotherapy ＋ permanent immunity therapy group and chemotherapy ＋ short term immunity therapy of large dose group,while the mean proportion of CD4^＋CD25^＋＋ regulatory T（CD4^＋CD25high regulatory T）cell all decreased,which showed statistical difference（P〈0.05）.The proportion of CD4^＋CD25^＋Treg cell increased in chemotherapy group（P=0.035） and chemotherapy ＋ permanent immunity therapy group（P=0.039）.The change of the proportion of CD4^＋CD25^＋Treg cell increased and CD4^＋CD25high Treg decreased did not show statistical significant in three groups,which was P=0.86,P=0.14.Conclusion Immunochemotherapy do not have an advantage over chemotherapy alone in the aspect of improving immune function in advanced esophageal cancer patients.

关 键 词：食管肿瘤 免疫疗法 抗肿瘤联合化疗方案 T淋巴细胞亚群 

分 类 号：R735.1[医药卫生—肿瘤]