白细胞介素-10对体外培养人牙囊细胞增殖和碱性磷酸酶活性的影响  

Effect of interleukin-10 on proliferation and activity of alkaline phosphatase of human dental follicle cells

作　　者：钱红 金作林 段银钟 顾泽旭 陈学鹏 毕迎春 

机构地区：[1]第四军医大学口腔医院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2008年第11期618-621,共4页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金(30400510)资助

摘　　要：目的:研究白细胞介素-10(Interleukin-10,IL-10)对体外培养人牙囊细胞增殖和牙囊细胞碱性磷酸酶活性的影响。方法:体外培养人牙囊细胞,取生长状态良好的第5代细胞,用四甲基偶氮唑蓝法(MTT法)检测IL-10对细胞增殖的作用;用碱性磷酸酶试剂盒检测IL-10及其抑制剂对细胞碱性磷酸酶活性的作用。结果:0、1、10、25、50、100ng/mLIL-10对人牙囊细胞的增殖影响无显著性差异(P>0.05);10ng/mLIL-10作用0、1、3、5、7d对人牙囊细胞的增殖影响无显著性差异(P>0.05)。10ng/mLIL-10作用3～7d降低人牙囊细胞的碱性磷酸酶活性(P<0.05),10ng/mLIL-10抑制剂作用5～7d增加人牙囊细胞的碱性磷酸酶活性(P<0.05)。结论:IL-10对人牙囊细胞的增殖无影响。IL-10降低人牙囊细胞的碱性磷酸酶活性,说明IL-10抑制人牙囊细胞向成骨方向分化。AIM： To study the effect ofinterleukin- 10 （IL- 10） on the proliferation of human dental folli- cle cells （HDFCs） and the effects of IL - 10 and its inhibitor on the activity of alkaline phosphatase （ALP） of HDFCs. METHODS： HDFCs of the 5th passage were cocuhured with IL - 10 for the assay of proliferation. In addition, cells were co -cultured with IL - 10 or its inhibitor for the assay of activity of ALP. RESULTS： 0, 1, 10, 25, 50 or 100ng/mL IL - 10 had no significant difference on the proliferation of HDFCs（ P 〉 0.05 ）. 10ng/mL IL - 10 acting for 0d, 1 d, 3 ds, 5 ds or 7ds had no significant difference on the proliferation of HDFCs （ P 〉 0.05 ）. 10ng/mL IL - 10 act- ing for 3 -7 days decreased the activity of ALP（P 〈0.05）. 10ng/mL IL- 10 inhibitor acting for 5 -7days increased the activity of ALP（ P 〈 0.05）. CONCLUSION ： IL - 10 had no effect on the proliferation of HDFCs. However, IL - 10 suppressed the activity of ALP of HDFCs, inhibiting their differentiation to osteoblasts.

关 键 词：牙囊细胞 白细胞介素-10 增殖 碱性磷酸酶 

分 类 号：R780.2[医药卫生—口腔医学]