曲安奈德对兔视网膜色素上皮细胞DNA的损伤作用  

Triamcinolone acetonide on DNA damage in rabbit retinal pigment epithelium

作　　者：刘玲华[1] 刘正[1] 杨明[1] 李伟军[1] 付小玻[1] 张晓梅[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院眼科分院,黑龙江省哈尔滨市150001

出　　处：《眼科新进展》2008年第12期898-900,904,共4页Recent Advances in Ophthalmology

基　　金：黑龙江省教育厅科学技术研究项目(编号:11531179);黑龙江省卫生厅科研课题(编号:2007-224)~~

摘　　要：目的观察曲安奈德(triamcinolone acetonide,TA)对兔视网膜色素上皮(retinal pigment epithelium,RPE)细胞DNA的损伤作用。方法应用单细胞凝胶电泳技术,分别测定0g.L-1、0.001g.L-1、0.01g.L-1、0.1g.L-1和1.0g.L-1TA作用于5组(空白对照组、Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组)兔RPE细胞24h后细胞DNA的损伤情况。结果兔RPE细胞在含有不同浓度TA的培养液中培养24h后,Ⅱ、Ⅲ和Ⅳ组的尾DNA含量、尾矩、Olive尾矩均高于空白对照组(10.40±2.83,9.72±3.45,12.73±3.38;12.24±4.34,10.45±5.99,12.72±5.07;17.37±5.00,17.62±5.95,20.02±5.72;P<0.05)。Ⅳ组的尾DNA含量、尾矩、Olive尾矩与Ⅰ组相比差异有统计学意义(P<0.05)。结论TA可导致兔RPE细胞DNA损伤且具有量效相关性。Objective To investigate the DNA damages of triamcinolone acetonide（TA） on rabbit retinal pigment epithelium（RPE）.Methods Five different mass concentrations of TA（0 g·L-1,0.001 g·L-1,0.01 g·L-1,0.1 g·L-1 and 1.0 g·L-1） were used to five groups,respectively,regarded as blank control group,groupⅠ,groupⅡ,groupⅢ and group Ⅳ for more than 24 hours,and DNA damages were evaluated by single cell gel electrophoresis.Results After cultured for 24 hours in different concentrations of TA culture medium,tail DNA contents,tail moment and Olive tail moment of groupⅡ,Ⅲ and Ⅳ of rabbit RPE were significantly higher than those of blank control group（10.40±2.83,9.72±3.45,12.73±3.38, 12.24±4.34,10.45±5.99,12.72±5.07, 17.37±5.00,17.62±5.95,20.02±5.72, P〈0.05）.Significant difference was found between the group Ⅳ and group Ⅰ（P〈0.05）.Conclusion TA can cause DNA damages on rabbit RPE in a dose-dependent manner.

关 键 词：曲安奈德 视网膜色素上皮 上皮细胞 单细胞凝胶电泳 

分 类 号：R774.1[医药卫生—眼科]