灵芝三萜抑制口腔黏膜癌发展过程的实验研究  

Experimental study of the inhibition of triterpenoids isolated from ganoderma lucidum on the development of oral squamous cell carcinoma

作　　者：张娟[1] 高文信[1] 王翔[2] 魏秀峰[1] 蔡研[1] 王家凤[1] 

机构地区：[1]吉林大学口腔医学院,130041 [2]南京市口腔医院

出　　处：《实用口腔医学杂志》2008年第6期784-788,共5页Journal of Practical Stomatology

基　　金：吉林省科技厅基金项目(编号:20050405-1)

摘　　要：目的:探讨灵芝三萜在口腔癌变发生发展过程中的抑制作用和机制。方法:建立金黄地鼠颊囊动态癌变的动物模型60只,分2组:A组(灵芝三萜组),B组(对照组),每组30只。采用免疫组化技术检测VEGF在2组动物模型中的表达变化,并进行统计学处理。结果:6周时A组正常黏膜上皮例数多于B组,上皮异常增生率A组明显低于B组(P<0.01),9周时中、重度上皮异常增生和癌变率A组低于B组(P<0.05),12周时A组癌变率低于B组(P<0.01)。在整个癌变动态观察期内,2组颊囊癌变过程中不同组织学发生情况有显著差异(P<0.01),A组病变严重程度始终低于B组。VEGF表达强度随着正常黏膜至鳞癌的发展逐渐增强。在上皮单纯增生、上皮异常增生、鳞癌中A组与B组相比,差异均有显著性(P<0.05)。结论:灵芝三萜对口腔黏膜癌发展过程中具有抑制作用并显示出明显的血管抑制作用。Objective ：To discuss the inhibition mechanism of Ganoderma triterpenoids on the development and progression of oral cancer. Methods ： 60 hamster cheek pouch dynamic cancer animal models were established and divided into two groups. A group was treated with Ganoderma triterpenoids, B group was as control. The expression of VEGF were detected with immunohistochemistry method. The data was analyzed with CS10.34. Results ： The pathological results showed that the normal epithelial cases of group A （ Ganoderma triterpenoids group） were more than those of group B（ the control group）and the epithelial dysplasia rate was significantly lower than group B（ P 〈 0.01 ） on 6th week. On 9th week the rates of moderate and severe epithelial dysplasia and carcinoma were lower in group A than in group B（ P 〈0.05）. On 12th week the cancer rate was significantly lower in group A than that in group B（P 〈0.01 ）. In the whole dynamic carcinogenesis observation period, the occurrence of two groups＇histological difference was significant（ P 〈0.01 ）. The severity in group A was still lower than that in group B and the grade of cancer induced was lower. Immunohistochemical study showed that the expression of VEGF gradually strengthened with the development of squamous cell carcinoma from normal mucosa. There were significant differences on the three grades, the simple hyperplasia, dysplasia and carcinoma information, of group A and B by rank sum test （ P 〈 0.05 ）. Conclusion： Ganoderma triterpenoids can inhibit carcinoma of the oral mueosa and showed significant inhibition of angiogenesis. It can be used for oral squamous cell carcinoma treatment.

关 键 词：灵芝三萜 口腔 鳞状细胞癌 血管上皮生长因子 抗肿瘤 

分 类 号：R739.8[医药卫生—肿瘤]