釉基质蛋白对人骨髓基质细胞增殖和矿化的影响  

Effects of EMPs on proliferation and mineralization of human BMSCs

作　　者：宋忠臣[1] 束蓉[1] 谢玉峰[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院牙周病科.上海市口腔医学研究所,200011

出　　处：《实用口腔医学杂志》2008年第6期831-834,共4页Journal of Practical Stomatology

基　　金：国家"863"计划基金项目(编号:2002AA205013);国家自然科学基金项目(编号:30672315;30801292);上海市高校优秀青年教师科研专项基金项目(编号:JDY07059)

摘　　要：目的:观察釉基质蛋白(enamelmatrix proteins,EMPs)对人骨髓基质细胞(bone marrow stromal cells,BMSCs)细胞周期和碱性磷酸酶(ALP)活性的影响。方法:体外培养人骨髓基质细胞,以200μg/ml EMPs作用于细胞为实验组,以不加EMPs为对照组。流式细胞仪检测EMPs对BMSCs细胞周期的影响,碱性磷酸酶试剂盒检测ALP的活性。结果:体外培养的人BMSCs生长良好,200μg/ml浓度的EMPs能提高细胞的ALP活性;影响细胞周期变化,EMPs组S期细胞数目较多,为19.48%,而对照组S期细胞数目为9.89%。结论:釉基质蛋白可促进人骨髓基质细胞的分化,影响细胞周期变化。Objective： To investigate the effects of enamel matrix proteins （EMPs） on cell cycle and alkaline phosphate （ALP） activity of human bone marrow stromal cells（BMSCs）. Methods：Human BMSCs were cultured in DMEM medium with 10% fetal bovine serum（FBS） in vitro. EMPs was added into medium at a concentration of 200μg/ml as experimental group. BMSCs were cultured without EMPs as control group. Cell cycle was evaluated by flow cytometry. ALP activity was also detected by ALP detection kit. Results： EMPs at a eoneentration of 200 μg/ml could enhance ALP activity and affect cell cycle of BMSCs. Cell number in S stage was 19.48% in EMPs group, and 9.89% in control group. Conelusion：EMPs can promote ALP activity of human BMSCs, and affect cell cycle of BMSCs. EMPs may act as a kind of promotive factor for BMSCs.

关 键 词：釉基质蛋白 骨髓基质细胞 增殖 矿化 细胞周期 

分 类 号：R781.4[医药卫生—口腔医学]