VCAM-1与TNF-α在口腔鳞癌血管生成中的协同作用  

Synergistic effect of vascular cell adhesion molecule-1 and tumor necrosis factor-α on angiogenesis in oral squamous cell carcinoma

作　　者：孙乐刚[1] 毛玉龙[1] 刘玲[2] 宋宇峰[3] 杨佑成[1] 王丽芳[1] 

机构地区：[1]山东滨州医学院附院口腔外科,256603 [2]山东滨州医学院附院胃镜室,256603 [3]贵阳医学院附院口腔外科

出　　处：《实用口腔医学杂志》2008年第6期863-866,共4页Journal of Practical Stomatology

基　　金：贵州省第二批跨世纪人才省政府专项基金项目(黔科合人专字2000-9813号);贵州省优秀科技教育人才省长专项基金项目(黔科教办[2004]07号)

摘　　要：目的:探讨血管细胞黏附分子-1(vascular cell adhesion molecule-1,VCAM-1)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)在口腔鳞癌中的表达及其在口腔鳞癌血管生成中的协同作用。方法:应用免疫组化二步法检测48例口腔鳞癌组织和10例正常口腔黏膜组织中VCAM-1、TNF-α和CD31表达并计数微血管密度(microvessel density,MVD)。结果:VCAM-1和TNF-α在口腔鳞癌组织的表达率明显高于正常口腔黏膜组织(P<0.01);VCAM-1和TNF-α同时表达阳性的肿瘤组织中MVD值明显高于VCAM-1和TNF-α单一表达阳性的MVD值(P<0.05)。结论:VCAM-1和TNF-α在口腔鳞癌组织中的高表达可能与肿瘤血管生成有关,并可能在血管生成中具有协同效应,共同促进肿瘤的生长和转移。Objective： To investigate the expression and the synergistic effect on angiogenesis of vascular cell adhesion molecule-1 （VCAM-1） and tumor necrosis factor-α （TNF-α）in oral squamous cell carcinoma （OSCC）. Methods： Expression of VCAM-1 and TNF-α and CD31 in 48 cases of OSCC and 10 cases of normal oral mucosa was detected by immunohistochemistry PV-9000 method. MVD was also assessed. Results：The expression rate of VCAM-1 and TNF-α was significantly higher in OSCC than those in normal oral mueosa（ P 〈 0.01 ）. MVD was significantly higher in VCAM-1 and TNF-α-positive OSCC than that in VCAM-1 or TNF-α-positive OSCC （P 〈 0.05）. Conclusion ： Over-expression of VCAM-1 and TNF-α in OSCC may be correlated with the angiogenesis and have an synergistic effeet on angiogenesis. They may promote the oral cancer growth and metastasis together.

关 键 词：口腔鳞癌 血管细胞黏附分子-1 肿瘤坏死因子-Α 血管生成 协同作用 

分 类 号：R739.8[医药卫生—肿瘤]