胶束毛细管电泳在线扫集技术同时分离测定穿心莲内酯和脱水穿心莲内酯的研究  

Study on separation and determination of dehydroandrogra-pholide and andrographolide in andrographis paniculata nees by sweeping micellar eletrokinetic capillary chromatography

作　　者：李利军[1] 吴峰敏[2,3] 喻来波[3] 冯军[3] 吴健玲[3] 孔红星[1] 

机构地区：[1]广西工学院生物与化学工程系,柳州545006 [2]河南科技大学化工与制药学院,洛阳471003 [3]广西大学化学与化学工程学院,南宁530005

出　　处：《分析试验室》2008年第12期55-58,共4页Chinese Journal of Analysis Laboratory

基　　金：广西自然科学基金(桂科自0481019);广西教育厅科学研究基金(桂教科[2000]392)项目资助

摘　　要：采用胶束毛细管电泳在线扫集技术分离测定了中药穿心莲中脱水穿心莲内酯和穿心莲内酯：电泳条件：以20mmol／LH3BO3—10mmol／LNaH2PO4—50mmol／LSDS（含体积分数20％甲醇，pH2．4）为电泳运行缓冲溶液，未涂层石英毛细管（58cm×50um i．d．，有效长度为41．2cm）为分离通道，重力进样，进样高度为11cm，-20kV恒压，检测波长为246nm。富集倍数可以达到200倍以上。在5．70～91．20mg／L，和3．96～31．68mg／L范围内呈良好的线性关系，对两种内酯分别进行了定量分析。加标平均回收率脱水穿心莲内酯为100．80％，穿心莲内酯为98．06％。Andrographis paniculata nees is one of the well-known traditional chinese medicinal materials, in which andrographolide and dehydroandrographolide are two major active compounds. In this work, a sweeping micellar electrokinetic capillary chromatographic method has been developed for separation and quantitative analysis of these two compounds in andrographis paniculata nees. Electrophoretic conditions are as follows： uncoated fused silica capillary （41.2 cm/58 cm, 50 tan i. d. ）, 20 mmol/L boric acid-10 mmol/L sodium dodecylsulfate-50 mmol/L SDS containing 20% methanol （ V/V） at pH 2.4, applied voltage - 20 kV, detection wave-length 240 nm, injection by gravity with the height 11 era. Up to 200-fold improvement in enrichment was achieved by using this sweeping injection technique. Both of the analytes were separated successfully within 10 min under the optimum conditions. The relative peak areas of the two analytes increase linearly with the increase of their concentrations in the range of 5.70 - 91.2 mg/L for dehydroandrographolide and 3.96 - 31.68 mg/L for andrographolide, and the average recoveries are 100.8% and 98.06%, respectively.

关 键 词：毛细管电泳 在线扫集 穿心莲 穿心莲内酯 脱水穿心莲内酯 

分 类 号：O657.8[理学—分析化学]