吉林省主推中晚熟期玉米品种DNA指纹库的构建  

Establishment of DNA Fingerprinting Database of Dominant Middle-late Mature Maize Hybrids in Jilin Province

作　　者：付玉芹[1] 隋光磊[1] 江源[1] 王丕武[1] 关淑艳[1] 刘树英[1] 胡欣[2] 

机构地区：[1]吉林农业大学生命科学学院,长春130118 [2]中国人民解放军总后勤部军需装备研究所,北京100010

出　　处：《吉林大学学报（理学版）》2008年第6期1221-1224,共4页Journal of Jilin University:Science Edition

基　　金：吉林省科技厅科研项目基金(批准号:20040203-2-3);吉林农业大学博士启动基金(批准号:200614)

摘　　要：以吉林省中晚熟期的43个玉米品种为材料,从玉米胚中提取基因组DNA并利用10对简单重复序列(SSR)引物进行扩增,扩增产物经质量分数为8%的聚丙烯凝胶电泳检测,获得了清晰稳定的图谱,初步建立了吉林省中晚熟期玉米43个品种的DNA指纹库.Forty-three dominant middle-late mature maize hybrids in Jilin Province were selected in this study, from which DNA was extracted and amplified with 10 pairs of simple sequence repeat （SSR） primers, the products were detected with 8% polyacrylamide gel electrophoresis. Clear and stable profiles of 43 samples were gained and the DNA fingerprinting database of 43 middle-late mature maize hybrids was established.

关 键 词：玉米 简单重复序列 DNA指纹 

分 类 号：Q75[生物学—分子生物学]