文蛤抗癌多肽抑制肝癌细胞SMMC-7721差异蛋白质组分析  

Differential Proteomic Analysis of Inhinbtion of Anti-cancer Peptide from the Mercenaria on Hepatocellular Carcinoma Cell Line SMMC-7721

作　　者：贺量[1] 范成成[1] 康劲翮[1] 张剑[1] 冷波[1] 石艳[1] 李文岗[2] 陈清西[1] 

机构地区：[1]厦门大学生命科学学院,教育部细胞生物学与肿瘤细胞工程重点实验室,福建厦门361005 [2]福建医科大学附属厦门第一医院,福建厦门361004

出　　处：《厦门大学学报（自然科学版）》2008年第A02期83-86,共4页Journal of Xiamen University：Natural Science

基　　金：厦门市科技计划项目(3502Z20063021);厦门市卫生局资助项目(WSK0602);厦门大学科技创新工程基金(XDKJCX20043001)资助

摘　　要：为了研究文蛤抗癌多肽对肝癌细胞抑制作用的蛋白表达差异,寻找并鉴定差异蛋白质,探讨多肽抑制肝癌细胞的作用机理,本文通过双向电泳、考马斯亮蓝染色、M elan ie 4双向软件分析、质谱技术(MALD I-TOF-MS)和数据库检索分析多肽作用SMMC-7721后的差异蛋白质.利用差异蛋白质组方法分析了抗癌多肽对肝癌细胞SMMC-7721抑制后的蛋白变化,找到差异点并进行了质谱鉴定(MALD I-TOF-MS).结果表明,通过对差异蛋白功能的分析,推测抗癌多肽可能引起了肝癌细胞的凋亡,为进一步的抗癌多肽抑制肝癌细胞作用的研究提供了理论基础.To research differenial expressions of proteins in response to effect of Anti-cancer peptide from the Mercenaria on hepatocellular carcinoma cell line SMMC-7721 and discuss the machanism of effect of anti-cancer peptide. Two dimensional polyacrylamide gel electrophoresis, coomassie brilliant blue staining, melanie 4 2DE software analysis, peptide mass fingerprinting based on matrix assisted laser desorption time of flight mass spectrometry（MALDI-TOF-MS） were used to analyze differential proteins between SMMC-7721 and SMMC-7721 treated with anti-peptide. Differential proteins were identified. Some of them were signal transducdon proteins such as Ras-rehted protein M-Ras and R.OR2 ,ER-elicited response protein GRP78 ,TCP-1-beta and PDIA5 ,nucleus Lamina protein Lamin A and other proteins such as alpha 1 type XⅢ collagen isoform 4, Alpha-enolase and Zinc finger protein 57. Fuction of these differential proteins shows that the anti-peptide maybe induce SMMC-7721 cell line to apoptosis. The research will be useful to further study of the effects ofanticancer peptide from Mercenaria on the SMMC-7721 cells.

关 键 词：肝癌 蛋白质组 质谱 文蛤 SMMC-7721 

分 类 号：Q516[生物学—生物化学]