三氧化二砷免疫蛋白微球对膀胱肿瘤的杀伤作用及机制探讨  

Killing effect and mechanism of arsenic trioxide-loaded albumin immuno-nanospheres on bladder tumor

作　　者：周洁[1] 郭晓云[1] 莫曾南[1] 曾甫清[2] 

机构地区：[1]广西医科大学第一附属医院,广西南宁530021 [2]华中科技大学同济医学院附属协和医院

出　　处：《山东医药》2008年第30期1-3,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30700836);中国博士后科学基金资助项目(20070420152)

摘　　要：目的探讨单抗BDI-1导向的三氧化二砷免疫蛋白微球[As2O3-(HAS-NS)-BDI-1]对膀胱肿瘤的杀伤作用及其机制。方法建立裸鼠膀胱肿瘤模型,经尿道置套管针膀胱灌注As2O3-(HAS-NS)-BDI-1。观察小鼠生存情况并记录生存时间。解剖显微镜下观察肿瘤生长和浸润情况及各器官毒性表现。TUNEL法检测肿瘤细胞凋亡情况。结果As2O3-(HAS-NS)-BDI-1组肿瘤生长及向周围浸润受到明显抑制(95.2%),且各器官毒性表现明显较As2O3组轻。As2O3-(HAS-NS)-BDI-1组小鼠膀胱肿瘤细胞凋亡率明显高于其他各组(P均<0.05)。结论As2O3-(HAS-NS)-BDI-1膀胱灌注可抑制原位膀胱肿瘤生长且不引起明显的全身不良反应,其作用机制与As2O3促进肿瘤细胞凋亡有关。Objective To identify the killing effect and mechanism of arsenic trioxide-loaded albumin immuno-nanospheres [ As2O3-（HAS-NS）-BDI-1 ] targeted with Manoclonal Antibody BDI-1 on bladder tumor. Methods A model of nude mouse bladder tumor xenografts was established.As2O3-（HAS-NS）-BDI-1 was poured into the bladder though urethra of female mouse with a trochar.Nude mouses general condition and survival time were recorded. Tumor growth and infiltration and toxicity manifestation of several organs were observed with microscope. TUNEL method was used to detected tumor cells apoptosis. Results Compare with the group poured with As2O3 ,tumor growth and infiltration were obviously inhibited in the group poured with As2O3-（ HAS-NS）- BDI-1 （95.2 % ）. However, toxicity manifestation of organs were insignificant. Bladder tumor cells apoptosis rate is higher in the groups poured with As2O3-（HAS-NS）-BDI-1 than other groups（ P 〈 0.05）.Conclusions As2O3-（HAS-NS）-BDI-1 intravesical instillation may inhibite bladder tumor growth and displayed insignificant total body toxicity effect. The effect mechanisms may be that As2O3 accelerate tumor cell apoptosis.

关 键 词：三氧化二砷 免疫蛋白微球 膀胱肿瘤 膀胱灌注 

分 类 号：R737.14[医药卫生—肿瘤]