华蟾素对卵巢癌3AO细胞CD44s表达的影响  

The Effect and Significance of Cinobufagin on the Expression of CD44s in Ovarian Carcinoma 3AO Cells

作　　者：王绍光[1] 姜学强[1] 

机构地区：[1]山东省烟台毓璜顶医院,烟台264000

出　　处：《国际中医中药杂志》2008年第6期405-406,共2页International Journal of Traditional Chinese Medicine

基　　金：山东省烟台市科委资助（项目编号：2005126）

摘　　要：目的 探讨华蟾素对体外培养的卵巢癌3AO细胞CD44s表达的影响。方法体外培养卵巢癌3AO细胞，不同浓度的华蟾素干预后，应用RT-PCR检测CD44smRNA的表达，流式细胞仪检测细胞的凋亡。结果卵巢癌3AO细胞对照组CD44smRNA表达为1．3±0．1，细胞凋亡率为（2．31±0．98）％；0．25mg／ml华蟾素对卵巢癌3AO细胞CD44smRNA和凋亡率的影响较对照组，无显著性差异（P〉0．05）；2．5mg／mL华蟾素干预后，3AO细胞CD44smRNA表达为1．0±0．1，癌细胞凋亡率为（28．69±4．16）％，较对照组有显著性差异（P〈0．05）；25mg·ml^-1华蟾素与2．5mg·ml^-1华蟾素对卵巢癌3AO细胞CD44smRNA表达和凋亡率的影响无显著性差异（P〉0．05），较对照组有显著性差异（P〈0．05）。结论2．5mg·ml^-1华蟾素可有效抑制卵巢癌3AO细胞CD44s的表达，抑制癌细胞的侵蚀性生长。Objective To investigate the effect and significance of cinobufagin on the expression of CD44s in ovarian carcinoma 3AO cells cultured in vitro. Methods Ovarian carcinoma 3AO cells were cultured in vitro; after the intervention of cinobufagin with different concentration, the expression of CD44s was measured by RT-PCR, and the cellular apoptosis was tested by flow cytometry. Results Ovarian carcinoma 3AO cells expressed CD44s mRNA of 1.3 ±0.1 and the cellular apoptotic rate was （2.31 ± 0.98）%1 0.25 mg/ml cinobufagin did not affect CD44s mRNA and cellular apoptofic rate （P 〉0.05） ; when 2.5 mg/ml cinobufagin was intervened , the expression of CD44s mRNA was 1.0±0.1 and the cellular apoptotic rate was （28.6±4.16）%, showing significant difference comparing with the control group of ovarian carcinoma 3AO cells （P〈0.05）; the intervention of 25 mg/ml cinobufagin was Similar to that of 0.25 mg/ml cinobufagin （P〉0.05）. Conclusion 2.5 mg · ml^-1 cinobufagin inhibit the expression of CD44s and the invasive growth of the ovarian carcinoma 3AO cells.

关 键 词：华蟾素 卵巢癌 CD44S 侵蚀性生长 

分 类 号：R737[医药卫生—肿瘤]