GlcNAcT在肿瘤^(99)Tc^m-DTPA-DG分子显像中的作用及意义  

The effectiveness and meaning of GlcNAcT in the mechanism of ^(99)Tc^m-DTPA-DG imaging

作　　者：孟晓云[1] 陈跃[1] 梁杰[1] 孙媛媛[1] 黄占文[1] 

机构地区：[1]泸州医学院附属医院,四川泸州646000

出　　处：《山东医药》2008年第41期5-7,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30670582)

摘　　要：目的探讨肿瘤99Tcm-DTPA-DG分子显像的相关机制中N乙酰氨基葡萄糖转移酶(GlcNAcT)的作用及意义。方法建立负肺癌雌性裸鼠模型,剥除肿瘤组织行HE染色并进行免疫荧光检测99Tcm-DTPA-DG在不同活度下GlcNAcT变化趋势。剥离的肿瘤组织匀浆后提取胞浆蛋白及细胞核蛋白,比较细胞核是否摄取TcO4-、99Tcm-DTPA、18F-FDG和99Tcm-DTPA-DG。结果肿瘤组织摄取不同活度99Tcm-DTPA-DG后,可见GlcNAcT蛋白表达有变化,GlcNAcT表达水平与99Tcm-DTPA-DG摄取率一致,有密切相关关系;99Tcm-DTPA-DG可被摄取进入细胞核且入核比率较其他药物高。结论99Tcm-DTPA-DG进入细胞后,可以入细胞核进一步参与细胞代谢,实现肿瘤分子显像,这与99Tcm-DTPA-DG在胞质内磷酸化后被GlcNAcT糖基化修饰有关。Objective To study the effectiveness and meaning of GlcNAcT in the mechanism of 99 Tc^m-DTPA-DG imaging. Methods The model of rat bearing lung cancer was constructed. The tumor tissue was been divested and stained by HE and fluorescent reagent. CPM was used to detect the quantity of ^99Tc^m-DTPA-DG uptaken in cell nucleus. Results The expression of GlcNAcT varies with the activity series of ^99Tc^m-DTPA-DG. It can be uptaken by cell nucleus. The upta- king rate is largest in TcO4- ,^99Tc^m-DTPA and 18FDG. Conclusions ~Tcm-DTPA-DG may inter cell nucleus and partici- pate in cell metabolism. The mechanism may be correlated with glycosylation of GlcNAcT.

关 键 词：肿瘤 ^99Tc^m-DTPA-DG GlcNAcT 分子显像 CPM计数 糖基化 

分 类 号：R730.44[医药卫生—肿瘤]