苦参碱对大鼠胶质瘤模型的作用的实验研究  

Study on Glioma Model Rats Treated by Matrine

作　　者：刘微[1] 邢春艳[2] 张淑君[3] 昝丽坤[1] 贾影[1] 滕国鑫[1] 戚基萍[1] 

机构地区：[1]黑龙江省哈尔滨医科大学第一临床学院,150001 [2]黑龙江省大庆龙南医院 [3]黑龙江省医院南岗分院

出　　处：《黑龙江医药》2008年第6期12-15,共4页Heilongjiang Medicine journal

基　　金：国家自然基金资助项目(30470661);黑龙江省自然科学基金项目(D2007-39)

摘　　要：目的:研究C6胶质瘤细胞在大鼠脑内成瘤后,苦参碱对其作用。方法:采用脑立体定向术建立胶质瘤大鼠模型,通过TUNEL及免疫组织化学方法检测苦参碱对胶质瘤大鼠模型肿瘤细胞的作用。结果:TUNEL及免疫组织化学Fas表达结果均显示在空白对照组与阴性对照组差异无统计学意义(P>0.05);低剂量组高于空白对照组和阴性对照组,差异有统计学意义(P<0.01);高剂量组高于低剂量组、阴性对照组和空白对照组,差异有统计学意义(F<0.01)。结论:苦参碱具有诱导体内胶质瘤细胞凋亡的作用.可能是通过增加Fas的表达来实现的。Objective： To investigate the expression of matrine in matrine - treated C6 glima model rats.Methods： To set up glioma model of rats to utilize stereotactic apparatus use TUNELdyeing tests and irnmunohistochemistry to detect the effection of rats treated by matrine.Results： TUNEL dyeing tests and immunohistochemistry of Fas expressed variously in different groups. There is no significant difference between untreated group and bomeol-treated group（P〉 0.05） .Fas was obviously up regulated in the high- dose matrine- treated group compared with the low-dose group（P〈 0.01） . The expression of Fas was higher in low-dose group than untreated group and bomeol-treated group（P〈 0.01） . Conclusion： Matrine can induce the vivo glioma - cells＇ apoptesis.It does possibly by upregulating the expression of Fas.

关 键 词：手参碱 C6胶质瘤细胞 凋亡 FAS 

分 类 号：R739.41[医药卫生—肿瘤]