PPARγ基因多态性与2型糖尿病合并脑梗死的相关性研究  

A population-based study of peroxisome proliferator-activated receptor-gamma gene polymorphism for type 2 diabetes mellitus complicated with cerebral infarction in Chinese Han

作　　者：高鹏[1] 吴江[2] 杜丹华[2] 郭晖[1] 赵节绪[2] 杨玉梅[2] 

机构地区：[1]吉林大学第一医院内分泌科,吉林长春130021 [2]吉林大学第一医院神经内科,吉林长春130021

出　　处：《中风与神经疾病杂志》2008年第5期534-536,共3页Journal of Apoplexy and Nervous Diseases

基　　金：卫生部部属医院临床学科重点项目(10183);吉林省科技厅青年基金资助项目(20080161);吉林省科技厅资助项目(20060413-1)

摘　　要：目的探讨PPARγ基因多态性与北方汉族人2型糖尿病合并脑梗死的关系。方法本研究共纳入791例受试对象,分成3组：健康对照组（NC）337例、单纯糖尿病组（T2DM）250例和糖尿病伴脑梗死组（T2DM＋CI）204例。以PPARγ基因rs1875796为遗传标记,应用多聚酶链-限制性片段长度多态性（PCR-RFLP）技术检测PPARγ基因rs1875796的基因型。结果女性T2DM＋CI组PPARγ基因rs1875796的C等位基因频率高于T2DM组、NC组（χ^2=6.672,P=0.010,OR=1.991,95%CI 1.176~3.358和χ^2=12.384,P〈0.0001,OR=2.499,95%CI 1.500~4.162）;女性T2DM＋CI组的CC＋CT基因型频率高于T2DM组、NC组（χ^2=4.656,P=0.031,OR=2.008,95%CI 1.006~3.782和χ^2=8.462,P=0.004,OR=2.486,95%CI 1.346~4.593）。经多因素回归分析调整了传统危险因素的影响后,女性T2DM＋CI组的CC＋CT基因型与T2DM组、NC组比较,差异仍有显著性（χ^2=5.770,P=0.016,OR=2.713,95%CI 1.206~6.126）。结论PPARγ基因可能与女性2型糖尿病患者罹患脑梗死的发生有关。Objective To investigate the genetic association between the PPARγ gene polymorphism and type 2 diabetes mellitus complicated with cerebral infarction. Methods 791 people were recruited into this study, and they were divided into three groups：NC（337） ,T2DM（250） and T2DM with CI（204）. SNP rs1875796,a C to T base change located in intron 4 of the gene, was used as a genetic marker. PCR-based restriction fragment length polymorphism analysis was ap- plied to genotype rs 1875796（ Hha I site）. Results The frequency of allele C was significantly higher in female in T2DM with CI patients than T2DM and NS patients（χ^2 =6. 672 ,P =0. 010 ,OR = 1. 991,95% CI 1. 176 - 3. 358 and χ^2 = 12. 384, P 〈0.0001 ,OR =2. 499,95% CI 1. 500 -4. 162）. And the frequency of CC ＋ CT genotype was also significantly higher in female T2DM with CI patients than NC patients （χ^2 = 4. 656,P = 0. 031, OR = 2. 008,95% CI 1. 006 -- 3. 782 and χ^2 = 8. 462, P = 0. 004, OR = 2. 486,95 % CI 1. 346 -- 4. 593 ）. Multiple factor regression analysis showed that the difference was still significant after adjusting the traditional risk factors. The frequency of allele and genotype showed no significance between male T2DM complicated with CI and male T2DM and NC patients. Conclusions The present study suggests that the PPART gene be likely to contribute to the etiology of type 2 diabetes mellitus complicated with cerebral infarction in female Chinese Hun.

关 键 词：脑梗死 PPARΓ基因 单核苷酸多态性(SNP) 2型糖尿病 

分 类 号：R743.32[医药卫生—神经病学与精神病学] R587.1[医药卫生—临床医学]